CAJ | 학술논문

目的：探讨血内毒素定量测定对革兰氏阴性菌败血症的诊断意义。方法：对临床可疑革兰氏阴性菌败血症的患者同时采取血标本及无热源污染血标本各一份，分别作细菌培养和血内毒素定量测定。血内毒素测定采用MB-80微生物快速动态检测系统进行测定，统计学分析比较两种方法的一致性。结果：革兰氏阴性菌生长组血内毒素含量（105.56±147.4pg/ml）与无细菌生长组（8.89±12.86pg/ml）比较有显著统计学差异（t=-7.059,P<0.001）。以20.0pg/ml作为血内毒素定量测定判断革兰氏阴性菌败血症的cutoff值，其准确率84%、灵敏度81.08%、特异度85%。结论：血浆内毒素对于早期判断革兰氏阴性菌败血症有一定意义，有利于临床合理应用抗菌药物、及时对症处理。
목적：탐토혈내독소정량측정대혁란씨음성균패혈증적진단의의。방법：대림상가의혁란씨음성균패혈증적환자동시채취혈표본급무열원오염혈표본각일빈，분별작세균배양화혈내독소정량측정。혈내독소측정채용MB-80미생물쾌속동태검측계통진행측정，통계학분석비교량충방법적일치성。결과：혁란씨음성균생장조혈내독소함량（105.56±147.4pg/ml）여무세균생장조（8.89±12.86pg/ml）비교유현저통계학차이（t=-7.059,P<0.001）。이20.0pg/ml작위혈내독소정량측정판단혁란씨음성균패혈증적cutoff치，기준학솔84%、령민도81.08%、특이도85%。결론：혈장내독소대우조기판단혁란씨음성균패혈증유일정의의，유리우림상합리응용항균약물、급시대증처리。
OBJECTIVE To study the Diagnosis significance of plasma endotoxin detecting of patient with gram negative bacil us infection. METHODS Concentration of blood plasma endotoxin were measured by Microbiology Kinetic Rapid Reader MB-80 within patients Suspected with gram negative bacil us infection. At the same time to detect blood culture .RESULTS Mean plasma entoxin of al negative patient was 8.89±12.86pg/ml.In gram negative bacil us infection group, the result was 105.56±147.4pg/ml .T-test analysis indicates the mean plasma entoxin of four groups have notable difference(t=-7.059,P<0.001). Based on the content of 20pg/ml, blood plasma endotoxin showed accurate rate ,sensitivity, specificity of 84%、81.08%、85% respectively.CONCULION Plasma endotoxin detecting is helping to estimate what kind of bacteria infected. It is helpful to select antibacterial and handle syndrome on time.