分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2013年
2期
223-228
,共6页
粮食%黄曲霉毒素%超高效液相色谱%免疫亲和柱%无衍生
糧食%黃麯黴毒素%超高效液相色譜%免疫親和柱%無衍生
양식%황곡매독소%초고효액상색보%면역친화주%무연생
建立了免疫亲和柱净化-超高效液相色谱法快速测定粮食中黄曲霉毒素(Aflatoxins,AF)的检测方法.样品经提取后,用免疫亲和柱净化、浓缩,Waters Acquity UPLC BEH C18色谱柱(50 mm×2.1 mm,1.7 um)分离.以甲醇-水(40∶60,V/V)为流动相,流速为0.2 mL/min,进样量为1μL,荧光检测器检测,激发波长为360 nm,发射波长为440 nm,无需衍生.黄曲霉毒素B1,B2,G1,G2的保留时间小于5min,从样品前处理到结果分析整个过程小于45 min.根据3倍信噪比的峰响应值,确定黄曲霉毒素(B1,B2,G1,G2)检出限分别为0.15,0.05,0.40,0.06 pg,4种毒素在0.4 ~ 60.0 pg,0.2~15.0 pg,1.5~ 60.0 pg和0.2~15.0 pg范围内分别呈线性相关,相关系数R2值分别为0.9999,0.9999,0.9998和0.9992;在小麦、玉米、稻谷3类样品中加标回收率为77.4% ~ 104.2%,精密度为1.8% ~ 8.9%.本方法无需衍生即可同时测定粮食中4种黄曲霉毒素,适用于粮食中黄曲霉毒素的快速定量测定.
建立瞭免疫親和柱淨化-超高效液相色譜法快速測定糧食中黃麯黴毒素(Aflatoxins,AF)的檢測方法.樣品經提取後,用免疫親和柱淨化、濃縮,Waters Acquity UPLC BEH C18色譜柱(50 mm×2.1 mm,1.7 um)分離.以甲醇-水(40∶60,V/V)為流動相,流速為0.2 mL/min,進樣量為1μL,熒光檢測器檢測,激髮波長為360 nm,髮射波長為440 nm,無需衍生.黃麯黴毒素B1,B2,G1,G2的保留時間小于5min,從樣品前處理到結果分析整箇過程小于45 min.根據3倍信譟比的峰響應值,確定黃麯黴毒素(B1,B2,G1,G2)檢齣限分彆為0.15,0.05,0.40,0.06 pg,4種毒素在0.4 ~ 60.0 pg,0.2~15.0 pg,1.5~ 60.0 pg和0.2~15.0 pg範圍內分彆呈線性相關,相關繫數R2值分彆為0.9999,0.9999,0.9998和0.9992;在小麥、玉米、稻穀3類樣品中加標迴收率為77.4% ~ 104.2%,精密度為1.8% ~ 8.9%.本方法無需衍生即可同時測定糧食中4種黃麯黴毒素,適用于糧食中黃麯黴毒素的快速定量測定.
건립료면역친화주정화-초고효액상색보법쾌속측정양식중황곡매독소(Aflatoxins,AF)적검측방법.양품경제취후,용면역친화주정화、농축,Waters Acquity UPLC BEH C18색보주(50 mm×2.1 mm,1.7 um)분리.이갑순-수(40∶60,V/V)위류동상,류속위0.2 mL/min,진양량위1μL,형광검측기검측,격발파장위360 nm,발사파장위440 nm,무수연생.황곡매독소B1,B2,G1,G2적보류시간소우5min,종양품전처리도결과분석정개과정소우45 min.근거3배신조비적봉향응치,학정황곡매독소(B1,B2,G1,G2)검출한분별위0.15,0.05,0.40,0.06 pg,4충독소재0.4 ~ 60.0 pg,0.2~15.0 pg,1.5~ 60.0 pg화0.2~15.0 pg범위내분별정선성상관,상관계수R2치분별위0.9999,0.9999,0.9998화0.9992;재소맥、옥미、도곡3류양품중가표회수솔위77.4% ~ 104.2%,정밀도위1.8% ~ 8.9%.본방법무수연생즉가동시측정양식중4충황곡매독소,괄용우양식중황곡매독소적쾌속정량측정.