山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
24期
1-3
,共3页
毛亚丽%韩纪举%王云%常欢%夏作理%赵晓民
毛亞麗%韓紀舉%王雲%常歡%夏作理%趙曉民
모아려%한기거%왕운%상환%하작리%조효민
血小板%聚集%血小板体系%致聚剂%血小板变形%光学法%大鼠%小鼠
血小闆%聚集%血小闆體繫%緻聚劑%血小闆變形%光學法%大鼠%小鼠
혈소판%취집%혈소판체계%치취제%혈소판변형%광학법%대서%소서
blood platelets%aggregation%platelet system%agonist%platelet shape change%optical method%mice%rats
目的:探讨血小板聚集初期形状改变与聚集的关系,并分析其影响因素。方法制备小鼠、大鼠富血小板血浆( PRP)和人洗涤血小板,以生理盐水、贫血小板血浆( PPP)或Tyrode液调整血小板计数来建立不同的血小板体系,二磷酸腺苷( ADP)或胶原诱导聚集,光学法测定血小板形状变化指标(最大负值和达最大负值时间)以及最大聚集率。结果人的最大负值、达最大负值时间比小鼠增加(P均<0.05),最大负值比大鼠增加(P<0.01);大鼠达最大负值时间比小鼠增加(P<0.01)。生理盐水稀释人PRP比PPP稀释人PRP最大负值减小,达最大负值时间延长(P均<0.05)。在人PRP中,胶原所致最大负值比ADP减小(P<0.05),达最大负值时间增加(P<0.01);在人洗涤血小板中,胶原所致最大负值、达最大负值时间均比ADP增大(P均<0.01);ADP和胶原在洗涤血小板中比PRP中的最大负值、达最大负值时间(除应用胶原变大外)减小(P均<0.01)。多元回归分析显示,聚集率与最大负值和达最大负值时间正相关(r分别为0.49、0.48,P均<0.01)。结论血小板聚集初期变形促进聚集,人的比大鼠、小鼠增强,生理盐水稀释PRP和洗涤血小板减弱,在PRP中胶原比ADP所致变形减弱,在洗涤血小板中增强。
目的:探討血小闆聚集初期形狀改變與聚集的關繫,併分析其影響因素。方法製備小鼠、大鼠富血小闆血漿( PRP)和人洗滌血小闆,以生理鹽水、貧血小闆血漿( PPP)或Tyrode液調整血小闆計數來建立不同的血小闆體繫,二燐痠腺苷( ADP)或膠原誘導聚集,光學法測定血小闆形狀變化指標(最大負值和達最大負值時間)以及最大聚集率。結果人的最大負值、達最大負值時間比小鼠增加(P均<0.05),最大負值比大鼠增加(P<0.01);大鼠達最大負值時間比小鼠增加(P<0.01)。生理鹽水稀釋人PRP比PPP稀釋人PRP最大負值減小,達最大負值時間延長(P均<0.05)。在人PRP中,膠原所緻最大負值比ADP減小(P<0.05),達最大負值時間增加(P<0.01);在人洗滌血小闆中,膠原所緻最大負值、達最大負值時間均比ADP增大(P均<0.01);ADP和膠原在洗滌血小闆中比PRP中的最大負值、達最大負值時間(除應用膠原變大外)減小(P均<0.01)。多元迴歸分析顯示,聚集率與最大負值和達最大負值時間正相關(r分彆為0.49、0.48,P均<0.01)。結論血小闆聚集初期變形促進聚集,人的比大鼠、小鼠增彊,生理鹽水稀釋PRP和洗滌血小闆減弱,在PRP中膠原比ADP所緻變形減弱,在洗滌血小闆中增彊。
목적:탐토혈소판취집초기형상개변여취집적관계,병분석기영향인소。방법제비소서、대서부혈소판혈장( PRP)화인세조혈소판,이생리염수、빈혈소판혈장( PPP)혹Tyrode액조정혈소판계수래건립불동적혈소판체계,이린산선감( ADP)혹효원유도취집,광학법측정혈소판형상변화지표(최대부치화체최대부치시간)이급최대취집솔。결과인적최대부치、체최대부치시간비소서증가(P균<0.05),최대부치비대서증가(P<0.01);대서체최대부치시간비소서증가(P<0.01)。생리염수희석인PRP비PPP희석인PRP최대부치감소,체최대부치시간연장(P균<0.05)。재인PRP중,효원소치최대부치비ADP감소(P<0.05),체최대부치시간증가(P<0.01);재인세조혈소판중,효원소치최대부치、체최대부치시간균비ADP증대(P균<0.01);ADP화효원재세조혈소판중비PRP중적최대부치、체최대부치시간(제응용효원변대외)감소(P균<0.01)。다원회귀분석현시,취집솔여최대부치화체최대부치시간정상관(r분별위0.49、0.48,P균<0.01)。결론혈소판취집초기변형촉진취집,인적비대서、소서증강,생리염수희석PRP화세조혈소판감약,재PRP중효원비ADP소치변형감약,재세조혈소판중증강。
Objective To investigate the relationship between initial platelet shape change and aggregation and to ana -lyze the influential factors .Methods After the platelet rich plasma ( PRP) in mice and rats and human washed platelets were prepared, platelet count was adjusted with normal saline (NS), platelet poor plasma (PPP) or Tyrode′s buffer.Neg-ative peak ( NP) and time to reach negative peak ( TNP) and maximum aggregation induced by adenosine monophosphate ( ADP) or collagen were determined by the turbidimetric method .Results Human NP and TNP were more than that of mice (all P<0.05), only human NP was more than that of rats (P<0.01), whereas rat TNP was more than that of mice (P<0.01).Human TP decreased and TNP prolonged significantly (all P<0.05) in PRP diluted with NS as compared with that diluted with PPP.Compared with ADP, collagen decreased NP (P<0.05) and increased TNP (P<0.01) in human PRP, and increased NP and TNP (P<0.01) in human washed platelets.Both ADP and collagen decreased NP and TNP in washed platelets than in PRP with the exception of increase in NP induced by collagen (all P<0.01).Multivariate regression analysis showed that aggregation was positively correlated with NP (r=0.49, P<0.01) and TNP (r=0.48, P<0.01).Conclusions Initial platelet shape change enhances aggregation .It is more in human than in rats and mice, is less in PRP diluted with NS and washed platelets , decreases in PRP with collagen and increases in washed platelets with ADP.