CAJ | 학술논문

目的：探讨大鼠妊娠早期暴露有机磷农药毒死蜱对子代鼠成年后大脑氧化损伤与机制。方法在母鼠妊娠后第6天至子鼠出生后第21天给予灌胃染毒，染毒剂量1/50LD50、1/100LD50、1/180LD50浓度的CPF和玉米油组。待子鼠长到第8周后处死，处死取脑做匀浆测大脑匀浆中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-PXD)活力。结果随着染毒剂量的增加，SOD的活力、GSH-PX的活力下降；而MDA的含量增加，并呈现出一定的剂量反应关系。各暴露染毒剂量组的上述指标还与玉米油对照组之间的差异有统计学意义(P＜0.05)。结论妊娠期毒死蜱暴露对8周龄子鼠大脑组织具有神经毒性，其机制可能与毒死蜱暴露至大脑氧化损伤有关。
목적：탐토대서임신조기폭로유궤린농약독사비대자대서성년후대뇌양화손상여궤제。방법재모서임신후제6천지자서출생후제21천급여관위염독，염독제량1/50LD50、1/100LD50、1/180LD50농도적CPF화옥미유조。대자서장도제8주후처사，처사취뇌주균장측대뇌균장중병이철(MDA)함량、초양화물기화매(SOD)활력、곡광감태과양화물매(GSH-PXD)활력。결과수착염독제량적증가，SOD적활력、GSH-PX적활력하강；이MDA적함량증가，병정현출일정적제량반응관계。각폭로염독제량조적상술지표환여옥미유대조조지간적차이유통계학의의(P＜0.05)。결론임신기독사비폭로대8주령자서대뇌조직구유신경독성，기궤제가능여독사비폭로지대뇌양화손상유관。
Objective To discuss whether there is any oxidative damage in the adult offspring rats’brain because of wistar rats exposed to organ phosphorus pesticide chlorpyrifos in early pregnancy.Methods Stomach virus infected dose of 1/50 LD50, 1/100 LD50 group, 1/180 LD50 concentration of CPF and corn oil were given to the mother rats intragastrically from the sixth day that the mother rats getting pregnancy to the twenty-first day after the rats born. The rats were put to death 8 weeks after born, then malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-PX) dynamic of brain were tested. Results As the increase of decreasing dose, the vigor of SOD and GSH-PX reduced;the content of MDA increased, and there was a dose-response relationship. There were statistically significant differences in the above indicators between every infected dose and corn oil control group (P<0.05).Conclusion There is certain oxidative damage on the brain of 8-week old rat adult offspring because of gestational exposure to low concentration chlorpyrifos.