计算机工程与应用
計算機工程與應用
계산궤공정여응용
COMPUTER ENGINEERING AND APPLICATIONS
2014年
8期
226-230,264
,共6页
优化%通量平衡分析%双层规划%对偶理论%代谢%1,3-丙二醇%甘油
優化%通量平衡分析%雙層規劃%對偶理論%代謝%1,3-丙二醇%甘油
우화%통량평형분석%쌍층규화%대우이론%대사%1,3-병이순%감유
optimization%flux balance analysis%bilevel programming%duality theory%metabolism%1,3-propanediol%glycerol
通量平衡分析是代谢工程研究中能够定量预测细胞内通量分布情况的有效工具，然而其预测准确性的前提是需要设计合理的代谢目标函数。研究了克雷伯氏杆菌歧化甘油生产1，3-丙二醇的代谢目标函数计算问题。该问题的数学形式是一个双层规划模型，首先应用线性规划的对偶理论将其转化为等价的单层非线性优化问题，然后采用非线性优化算法对其进行求解。与已有的研究结果相比，所提方法获得了克雷伯氏杆菌歧化甘油代谢的全局最优通量分布情况。
通量平衡分析是代謝工程研究中能夠定量預測細胞內通量分佈情況的有效工具，然而其預測準確性的前提是需要設計閤理的代謝目標函數。研究瞭剋雷伯氏桿菌歧化甘油生產1，3-丙二醇的代謝目標函數計算問題。該問題的數學形式是一箇雙層規劃模型，首先應用線性規劃的對偶理論將其轉化為等價的單層非線性優化問題，然後採用非線性優化算法對其進行求解。與已有的研究結果相比，所提方法穫得瞭剋雷伯氏桿菌歧化甘油代謝的全跼最優通量分佈情況。
통량평형분석시대사공정연구중능구정량예측세포내통량분포정황적유효공구，연이기예측준학성적전제시수요설계합리적대사목표함수。연구료극뢰백씨간균기화감유생산1，3-병이순적대사목표함수계산문제。해문제적수학형식시일개쌍층규화모형，수선응용선성규화적대우이론장기전화위등개적단층비선성우화문제，연후채용비선성우화산법대기진행구해。여이유적연구결과상비，소제방법획득료극뢰백씨간균기화감유대사적전국최우통량분포정황。
Flux balance analysis is an effective tool that can quantitatively predict the flux distribution of cellular metabo-lism in the study of metabolic engineering. The accuracy of this kind of predictions can be achieved by computing a rea-sonable objective function. This paper considers the problem of computing an objective function of glycerol metabolism in Klebsiella pneumoniae for 1, 3-propanediol production. The mathematical representation of this problem is a bilevel programming model. The dual theory of linear programming is firstly used to transform the above bilevel programming into an equivalent nonlinear optimization problem. Then the obtained problem can be solved by nonlinear optimization algo-rithms. Compared with the current method, the proposed approach obtains the globally optimal flux distribution of glycerol metabolism in Klebsiella pneumoniae for 1, 3-propanediol production.
