中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2014年
13期
120-120,122
,共2页
高效毛细管电泳%含量测定%头孢噻吩%头孢拉啶
高效毛細管電泳%含量測定%頭孢噻吩%頭孢拉啶
고효모세관전영%함량측정%두포새분%두포랍정
High performance capillary electrophoresis%Determination of content%Cephalothin%Cephradine
目的：研究高效毛细管电泳同时测定头孢噻吩和头孢拉啶的结果分析。方法：采用高效毛细管电泳法对头孢噻吩和头孢拉啶分别进行测定，以甲醇为有机添加剂，在进样前毛细管清洗顺序为；HCL 溶液，二次蒸馏水， NaOH溶液，二次蒸馏水，缓冲液冲洗。在两次进样测定间隙要用缓冲液冲洗平衡5分钟。通过各种参数的考察确定电泳条件。结果：电泳分离模式电泳条件20mmol/L硼砂缓冲液，分离电压18kV，柱温25℃，进样40秒，pH 6.0，检测波长280nm；有效长度40cm，运行液中添加20mmol/L的SDS和体积分数25%甲醇。结论：优化后的电泳运行缓冲液背景良好，检出限稳定，精密度高，且实验操作简单，取样量少分析成本低，为快速分离和检测多种头孢菌素类抗生素提供了一种有效方法，值得广泛推广。
目的：研究高效毛細管電泳同時測定頭孢噻吩和頭孢拉啶的結果分析。方法：採用高效毛細管電泳法對頭孢噻吩和頭孢拉啶分彆進行測定，以甲醇為有機添加劑，在進樣前毛細管清洗順序為；HCL 溶液，二次蒸餾水， NaOH溶液，二次蒸餾水，緩遲液遲洗。在兩次進樣測定間隙要用緩遲液遲洗平衡5分鐘。通過各種參數的攷察確定電泳條件。結果：電泳分離模式電泳條件20mmol/L硼砂緩遲液，分離電壓18kV，柱溫25℃，進樣40秒，pH 6.0，檢測波長280nm；有效長度40cm，運行液中添加20mmol/L的SDS和體積分數25%甲醇。結論：優化後的電泳運行緩遲液揹景良好，檢齣限穩定，精密度高，且實驗操作簡單，取樣量少分析成本低，為快速分離和檢測多種頭孢菌素類抗生素提供瞭一種有效方法，值得廣汎推廣。
목적：연구고효모세관전영동시측정두포새분화두포랍정적결과분석。방법：채용고효모세관전영법대두포새분화두포랍정분별진행측정，이갑순위유궤첨가제，재진양전모세관청세순서위；HCL 용액，이차증류수， NaOH용액，이차증류수，완충액충세。재량차진양측정간극요용완충액충세평형5분종。통과각충삼수적고찰학정전영조건。결과：전영분리모식전영조건20mmol/L붕사완충액，분리전압18kV，주온25℃，진양40초，pH 6.0，검측파장280nm；유효장도40cm，운행액중첨가20mmol/L적SDS화체적분수25%갑순。결론：우화후적전영운행완충액배경량호，검출한은정，정밀도고，차실험조작간단，취양량소분석성본저，위쾌속분리화검측다충두포균소류항생소제공료일충유효방법，치득엄범추엄。
Objective:Determination of cephalothin and cephradine for high performance capillary electrophoresis results. Methods:Cephalothin and cephradine were determined by high performance capillary electrophoresis,with methanol as the organic additives in capillary,before cleaning the order;HCL solution,two times in distilled water,NaOH solution,two times in distilled water,washing buffer.In the two sample clearance to rinsing with buffer balance for 5 minutes.Through the investigation of various parameters of electrophoresis conditions.Results:The conditions of electrophoresis separation mode 20mmol/L borax buffer electrophoresis,separation voltage of 18KV,the column temperature was 25℃ ,sampling 40 seconds,pH6.0,detection wavelength was 280nm;the effective length of 40cm,add 20mmol/L of SDS and 25% volume fraction of methanol in liquid operation.Conclusion:The electrophoretic running buffer background to optimize the detection limit,stability,high precision,and the experiment has the advantages of simple operation,less sample volume and low cost,provides an effective method for rapid separation and detection of several cephalosporins,worthy of extensive promotion.
