CAJ | 학술논문

用循环伏安法制备了银掺杂聚L-赖氨酸修饰玻碳电极,并对修饰电极表面进行了表征,研究了黄嘌呤和尿酸在该修饰电极上的电化学行为,建立了循环伏安法和示差脉冲伏安法同时测定黄嘌呤和尿酸的新方法。研究表明,修饰电极对尿酸和黄嘌呤的氧化具有较好的电催化活性,在pH 3.0磷酸盐缓冲溶液中,黄嘌呤和尿酸在银掺杂聚L-赖氨酸修饰电极上的氧化峰电位分别为0.980和0.600 V,两者的峰电位差达0.380 V。在最优实验条件下,用差分脉冲伏安法同时测定黄嘌呤和尿酸的线性范围为1.0×10-6~2.5×10-4 mol/L,检出限为5.0×10-7 mol/L。用于人尿样中黄嘌呤和尿酸的测定,获得满意结果。
용순배복안법제비료은참잡취L-뢰안산수식파탄전겁,병대수식전겁표면진행료표정,연구료황표령화뇨산재해수식전겁상적전화학행위,건립료순배복안법화시차맥충복안법동시측정황표령화뇨산적신방법。연구표명,수식전겁대뇨산화황표령적양화구유교호적전최화활성,재pH 3.0린산염완충용액중,황표령화뇨산재은참잡취L-뢰안산수식전겁상적양화봉전위분별위0.980화0.600 V,량자적봉전위차체0.380 V。재최우실험조건하,용차분맥충복안법동시측정황표령화뇨산적선성범위위1.0×10-6~2.5×10-4 mol/L,검출한위5.0×10-7 mol/L。용우인뇨양중황표령화뇨산적측정,획득만의결과。
The silver doped poly ( L-lysine ) modified glassy carbon electrode was fabricated by cyclic voltammetry, the surface of the electrode was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrochemical behaviors and the simultaneous detection of xanthine and uric acid were studied by cyclic voltammetry and differential pulse voltammetry. The results indicated that this modified electrode exhibited excellent electrocatalytic activity towards the oxidation of xanthine and uric acid. The stable oxidation peaks of xanthine and uric acid appeared with the peak potential of 0. 980V and 0. 600V respectively at the modified electrode in pH 3. 0 phosphate buffer solution. The oxidation peaks of xanthine and uric acid were separated at 380 mV. Under the optimum conditions, the linear ranges for the determination of xanthine and uric acid were 1 . 00 × 10-6-2 . 50 × 10-4 mol/L respectively by differential pulse voltammetry. The detection limits were 5. 0×10-7mol/L. The method has been applied to the simultaneous detection of xanthine and uric acid in healthy human urine with satisfactory results.