CAJ | 학술논문

在金电极表面,通过 Au-S 键接上具有“茎-环”结构的发夹适配子探针( Hairpin aptamer probe, HAP)；当加入Pb2+时,促使发夹结构适配子的“茎-环”结构打开,形成G-4联体,暴露出的3'-NH2与CdS量子点( QDs)结合,导致电化学发光,据此可构建测定Pb2+的量子点电化学发光“Turn on”型传感器。对电化学发光传感器进行了表征。在2.0×10-10~5.0×10-8 mol/L范围内,Pb2+浓度与其响应的ECL信号呈线性关系,检出限为3.74×10-11 mol/L。传感器有良好的选择性、稳定性及重现性。
재금전겁표면,통과 Au-S 건접상구유“경-배”결구적발협괄배자탐침( Hairpin aptamer probe, HAP)；당가입Pb2+시,촉사발협결구괄배자적“경-배”결구타개,형성G-4련체,폭로출적3'-NH2여CdS양자점( QDs)결합,도치전화학발광,거차가구건측정Pb2+적양자점전화학발광“Turn on”형전감기。대전화학발광전감기진행료표정。재2.0×10-10~5.0×10-8 mol/L범위내,Pb2+농도여기향응적ECL신호정선성관계,검출한위3.74×10-11 mol/L。전감기유량호적선택성、은정성급중현성。
A "turn on" model of electrochemiluminescence ( ECL)-Pb2+ biosensor based on G-quadruplex of aptamer probe was developed by using quantum dots ( QDs) as labels. The ECL-Pb2+ biosensor was prepared by immobilizing a hairpin aptamer probe with a thiol group at 5 '-end and an amino group at 3 '-end on gold electrode via an Au-S bond. In the presence of Pb2+, the G-rich hairpin aptamer probe opened the stem-loop and formed G-quadruplex, which exposed the amino group at 3'-end and then covalently linked to the carboxyl group on the surface of CdS quantum dots. The fabrication and reaction process of the Pb2+ biosensor were characterized. The responsive ECL intensity was linearly related to the Pb2+ concentration in the range of 2. 0×10-10-5. 0×10-8 mol/L, with the detection limit of 3. 74×10-11 mol/L. In addition, the ECL biosensor exhibited high sensitivity, good selectivity, satisfying stability, and repeatability.