中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2014年
20期
127-128
,共2页
淋球菌%沙眼衣原体%免疫层析法%培养法
淋毬菌%沙眼衣原體%免疫層析法%培養法
림구균%사안의원체%면역층석법%배양법
Neisseria gonorrhoeae%Chlamydia trachomatis%Immunochromatographic assay%Culture method
目的:探讨淋球菌/沙眼衣原体抗原二联检测试剂盒(乳胶免疫层析法)(以下简称淋球菌/衣原体二联检测试剂)检测女性宫颈分泌物中淋球菌/沙眼衣原体的临床应用。方法:收治疑为淋球菌/沙眼衣原体女性感染者558例,对其宫颈分泌物标本进行检测。淋球菌以培养法为金标准,沙眼衣原体以杭州艾康沙眼衣原体免疫层析试剂为对照,不符的标本用PCR确认,进行评价淋球菌/衣原体二联检测试剂的临床性能。结果:淋球菌/衣原体二联检测试剂检测淋球菌和沙眼衣原体的敏感性分别为96.88%和94.12%,特异性分别为95.75%和99.15%,准确性分别为95.88%和98.39%。结论:淋球菌/衣原体二联检测试剂操作简单、快速、具有较高的特异性、敏感性和有效性,适宜基层计生站孕前检测和妇保院普查应用。
目的:探討淋毬菌/沙眼衣原體抗原二聯檢測試劑盒(乳膠免疫層析法)(以下簡稱淋毬菌/衣原體二聯檢測試劑)檢測女性宮頸分泌物中淋毬菌/沙眼衣原體的臨床應用。方法:收治疑為淋毬菌/沙眼衣原體女性感染者558例,對其宮頸分泌物標本進行檢測。淋毬菌以培養法為金標準,沙眼衣原體以杭州艾康沙眼衣原體免疫層析試劑為對照,不符的標本用PCR確認,進行評價淋毬菌/衣原體二聯檢測試劑的臨床性能。結果:淋毬菌/衣原體二聯檢測試劑檢測淋毬菌和沙眼衣原體的敏感性分彆為96.88%和94.12%,特異性分彆為95.75%和99.15%,準確性分彆為95.88%和98.39%。結論:淋毬菌/衣原體二聯檢測試劑操作簡單、快速、具有較高的特異性、敏感性和有效性,適宜基層計生站孕前檢測和婦保院普查應用。
목적:탐토림구균/사안의원체항원이련검측시제합(유효면역층석법)(이하간칭림구균/의원체이련검측시제)검측녀성궁경분비물중림구균/사안의원체적림상응용。방법:수치의위림구균/사안의원체녀성감염자558례,대기궁경분비물표본진행검측。림구균이배양법위금표준,사안의원체이항주애강사안의원체면역층석시제위대조,불부적표본용PCR학인,진행평개림구균/의원체이련검측시제적림상성능。결과:림구균/의원체이련검측시제검측림구균화사안의원체적민감성분별위96.88%화94.12%,특이성분별위95.75%화99.15%,준학성분별위95.88%화98.39%。결론:림구균/의원체이련검측시제조작간단、쾌속、구유교고적특이성、민감성화유효성,괄의기층계생참잉전검측화부보원보사응용。
Objective:To explore the clinical application of gonorrhea/chlamydia trachomatis antigen two combined detection kit(latex immunochromatographic assay) (hereinafter referred to as gonococcal/chlamydia two joint detection reagent) in the detection of female cervical secretions.Methods:558 cases with suspected gonorrhea/chlamydia trachomatis infected females were selected.We detected the cervical secretion.The gold standard of neisseria gonorrhoeae was culture method.The control of chlamydia trachomatis was Hangzhou ACON chlamydia trachomatis immunochromatographic reagent.Discrepant samples were confirmed by PCR.We evaluated the clinical performance of gonococcal/chlamydia two joint detection reagent.Results:The sensitivity of gonococcal/chlamydia two joint detection reagent in the detection of neisseria gonorrhoeae and chlamydia trachomatis was 96.88% and 94.12% respectively.Specificity was 95.75% and 99.15% respectively.Accuracy was 95.88% and 98.39% respectively.Conclusion:Operation of gonococcal/chlamydia two joint detection reagent is simple and fast.It has the advantages of high specificity, sensitivity and effectiveness.So it is suitable for the pregnancy test in grassroots station and census application in the maternal and child health hospital.