现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
22期
2405-2408
,共4页
李葆青%邱模炎%李楠%吴鋆%刘恩%张万强%焦强%栾洁%王爱强%汪用文%王瑾
李葆青%邱模炎%李楠%吳鋆%劉恩%張萬彊%焦彊%欒潔%王愛彊%汪用文%王瑾
리보청%구모염%리남%오윤%류은%장만강%초강%란길%왕애강%왕용문%왕근
清热化湿%活血泻浊%大鼠%5/6肾切除%肾小球硬化
清熱化濕%活血瀉濁%大鼠%5/6腎切除%腎小毬硬化
청열화습%활혈사탁%대서%5/6신절제%신소구경화
clearing heat and removing dampness%promoting blood circulation purging turbid%rats%5/6 nephrectomy%glomerular sclerosis
目的：观察清热化湿、活血泻浊法对肾小球硬化大鼠肾组织病理的影响。方法将大鼠分为正常组、假手术组、模型组、尿毒清组、经验方组，后3组采用5/6肾切除法制作肾小球硬化大鼠模型，并给予相应药物灌胃4周，取肾组织切片，行HE、PAS染色，观察大鼠肾脏病理变化，计算肾小球硬化指数（ GSI ）；采用数码医学图像分析系统求得基质阳性面积比值。结果与正常组及假手术组比较，模型组GSI、基质阳性面积比值均升高（ P均＜0.05）。与模型组比较，经验方组GSI、基质阳性面积比值有降低（ P均＜0．05）；而尿毒清组差异无统计学意义（P＞0．05）。结论清热化湿、活血泻浊组成的经验方能够改善模型大鼠肾脏病理，降低GSI及基质阳性面积比值，改善肾小球硬化的程度。
目的：觀察清熱化濕、活血瀉濁法對腎小毬硬化大鼠腎組織病理的影響。方法將大鼠分為正常組、假手術組、模型組、尿毒清組、經驗方組，後3組採用5/6腎切除法製作腎小毬硬化大鼠模型，併給予相應藥物灌胃4週，取腎組織切片，行HE、PAS染色，觀察大鼠腎髒病理變化，計算腎小毬硬化指數（ GSI ）；採用數碼醫學圖像分析繫統求得基質暘性麵積比值。結果與正常組及假手術組比較，模型組GSI、基質暘性麵積比值均升高（ P均＜0.05）。與模型組比較，經驗方組GSI、基質暘性麵積比值有降低（ P均＜0．05）；而尿毒清組差異無統計學意義（P＞0．05）。結論清熱化濕、活血瀉濁組成的經驗方能夠改善模型大鼠腎髒病理，降低GSI及基質暘性麵積比值，改善腎小毬硬化的程度。
목적：관찰청열화습、활혈사탁법대신소구경화대서신조직병리적영향。방법장대서분위정상조、가수술조、모형조、뇨독청조、경험방조，후3조채용5/6신절제법제작신소구경화대서모형，병급여상응약물관위4주，취신조직절편，행HE、PAS염색，관찰대서신장병리변화，계산신소구경화지수（ GSI ）；채용수마의학도상분석계통구득기질양성면적비치。결과여정상조급가수술조비교，모형조GSI、기질양성면적비치균승고（ P균＜0.05）。여모형조비교，경험방조GSI、기질양성면적비치유강저（ P균＜0．05）；이뇨독청조차이무통계학의의（P＞0．05）。결론청열화습、활혈사탁조성적경험방능구개선모형대서신장병리，강저GSI급기질양성면적비치，개선신소구경화적정도。
Objective It is to observe the effect of clearing heat and removing dampness and removing promoting blood cir -culation purging turbid on rat renal glomerular sclerosis tissue pathology .Methods The rats were randomly divided into normal group, sham-operated group, model group, Niaoduqing group, the rat models of renal glomerular sclerosis were established in the latter three group by 5/6 nephrectomy .All the groups were given experimental prescription respectively for 4 weeks by intragastric administration , then section of renal tissue was made to do HE and PAS staining , kidney pathological changes of the rats were observed and glomerular sclerosis index ( GSI) was calculated;matrix positive area ratio was obtained using digit-al medical image analysis system .Results Compared with normal group and sham-operated group , in model group GSI , the matrix positive area ratio increased, the difference was statistically significant (P<0.05).Party group compared with model group, experimental prescription group GSI , matrix positive area ratio were lower , the difference was statistically significant (P<0.05); And no significant differences was found in Niaoduqing group (P>0.05).Conclusion The experiential pre-scription of method of clearing heat and removing dampness and promoting blood circulation purging turbid could improve mod -el rat renal pathology , reduce glomerular sclerosis index and positive area ratio , improve the degree of glomerular sclerosis .
