CAJ | 학술논문

目的：评价酶联免疫吸附试验(ELISA)和环介导等温扩增方法(loop-mediated isothermal amplification，LAMP)在检测妊娠期女性沙眼衣原体(Chlamydiae trachomatis，Ct)感染中的应用价值。方法：根据Genebank公布的Cpn基因序列设计6条 LAMP 引物(2条内引物，2条外引物，2条环引物)，优化并建立 LAMP 检测体系。收治 Ct 感染患者80例，进行LAMP法检测Ct DNA，同时用ELISA检测Ct特异性脂多糖抗原。结果：80例患者中，ELISA检测出阳性46例(57.5%)，而LAMP检测出阳性56例(70.0%)，两者比较差异无统计学意义(P＞0.05)。80例患者中有51例服用过大环内酯类抗生素，ELISA 检测出阳性26例(50.9%)，而 LAMP 检测出39例(76.5%)，两者比较差异有统计学意义(P＜0.05)。结论：LAMP检测泌尿生殖道Ct感染方法简便、快捷，尤其是对服用过抗生素的患儿也具有较高的检出率。
목적：평개매련면역흡부시험(ELISA)화배개도등온확증방법(loop-mediated isothermal amplification，LAMP)재검측임신기녀성사안의원체(Chlamydiae trachomatis，Ct)감염중적응용개치。방법：근거Genebank공포적Cpn기인서렬설계6조 LAMP 인물(2조내인물，2조외인물，2조배인물)，우화병건립 LAMP 검측체계。수치 Ct 감염환자80례，진행LAMP법검측Ct DNA，동시용ELISA검측Ct특이성지다당항원。결과：80례환자중，ELISA검측출양성46례(57.5%)，이LAMP검측출양성56례(70.0%)，량자비교차이무통계학의의(P＞0.05)。80례환자중유51례복용과대배내지류항생소，ELISA 검측출양성26례(50.9%)，이 LAMP 검측출39례(76.5%)，량자비교차이유통계학의의(P＜0.05)。결론：LAMP검측비뇨생식도Ct감염방법간편、쾌첩，우기시대복용과항생소적환인야구유교고적검출솔。
Objective:To evaluate the application value of enzyme linked immunosorbent assay(ELISA) and loop mediated isothermal amplification(LAMP) in the detection of women chlamydiae trachomatis(Ct) infection during pregnancy.Methods:6 LAMP primers(2 inner primers,2 outer primers,2 loop primers) were designed according to the sequence of Cpn gene published by Genebank.LAMP detection system was optimized and established.80 cases with Ct were selected.Ct DNA was detected by LAMP. Ct specific fat polysaccharide antigen was detected by ELISA.Results:In 80 cases,ELISA detected that 46 cases(57.5%) were positive,while LAMP detected that 56 cases(70%) were positive,and there was no statistical significance(P>0.05).In 80 cases,51 cases were treated with macrolide antibiotics;ELISA detected that 26 cases(50.9%) were positive,while LAMP detected that 39 cases(76.5%) were positive,and there was statistical significance(P<0.05).Conclusion:LAMP detection of urogenital Ct infection method is simple and fast, especially it has higher detection rate for the children with taking antibiotics.