CAJ | 학술논문

目的：探讨连翘酯苷对顺铂致豚鼠耳毒性的影响及其机制。方法30只豚鼠随机分为对照组、顺铂组和连翘酯苷组，每组各10只。顺铂组豚鼠腹腔注射顺铂溶液8 mg/（ kg· d），连续7 d；连翘酯苷组在每次注射顺铂溶液30 min前腹腔注射连翘酯苷25．0 mg/（kg· d），连续7 d；正常对照组以生理盐水代替顺铂溶液腹腔注射，连续7 d。实验动物被处死前，检测其畸变产物耳声发射（ DPOAE）幅值变化；采用免疫组化SP法和Western blot法检测各组豚鼠耳蜗组织bcl-2蛋白的表达。结果1、2、4、6、8 kHz测试频率下，3组DPOAE幅值比较差异均有统计学意义（P均＜0．05），即顺铂组＜连翘酯苷组＜对照组。3组豚鼠耳蜗组织bcl-2蛋白表达比较差异均有统计学意义（P＜0．05或＜0．01），即顺铂组＜连翘酯苷组＜对照组。结论连翘酯苷可减轻顺铂所致耳蜗损伤，上调耳蜗组织bcl-2蛋白表达可能是其作用机制之一。
목적：탐토련교지감대순박치돈서이독성적영향급기궤제。방법30지돈서수궤분위대조조、순박조화련교지감조，매조각10지。순박조돈서복강주사순박용액8 mg/（ kg· d），련속7 d；련교지감조재매차주사순박용액30 min전복강주사련교지감25．0 mg/（kg· d），련속7 d；정상대조조이생리염수대체순박용액복강주사，련속7 d。실험동물피처사전，검측기기변산물이성발사（ DPOAE）폭치변화；채용면역조화SP법화Western blot법검측각조돈서이와조직bcl-2단백적표체。결과1、2、4、6、8 kHz측시빈솔하，3조DPOAE폭치비교차이균유통계학의의（P균＜0．05），즉순박조＜련교지감조＜대조조。3조돈서이와조직bcl-2단백표체비교차이균유통계학의의（P＜0．05혹＜0．01），즉순박조＜련교지감조＜대조조。결론련교지감가감경순박소치이와손상，상조이와조직bcl-2단백표체가능시기작용궤제지일。
Objective To study the effect of forsythiaside on cisplatin-induced ototoxicity of guinea pigs .Methods Thirty guinea pigs were randomly divided into the control group (n=10), cisplatin group (n=10) and forsythiaside group (n=10).The guinea pigs in the cisplatin group were induced by intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days, the guinea pigs in the forsythiaside group were given forsythiaside (25 mg/kg per day) at 30 min be-fore cisplatin solution for 7 consecutive days , and the normal saline instead of cisplatin was injected in the control group . The distortion product otoacoustic emission ( DPOAE) was detected before the experimental animals were killed , and the expression of bcl-2 in cochlea of guinea pigs was detected by immunohistochemistry and Western blotting .Results Statis-tical difference was found in the DPOAE amplitudes under 1, 2, 4, 6 and 8 kHz test frequencies among the three groups:cisplatin group <forsythiaside group <control group (all P<0.05).Meanwhile, statistical difference was found in the expression of bcl-2 protein among the three groups:cisplatin group <forsythiaside group <control group (P<0.05 or P<0.01).Conclusion Forsythiaside can significantly reduce the cisplatin-induced ototoxicity by up-regulating the expres-sion of bcl-2.