CAJ | 학술논문

荧光素修饰的凝血酶适配体(5′-FAM-GGT TGG TGT GGT TGG-3′)可与Pb2﹢选择性地形成G-四链体，这一构象变化能改变作为能量供体的荧光染料和作为能量受体的金纳米粒子之间的距离，使体系的荧光强度与Pb2﹢浓度具有一定的相关性，从而建立了一种基于纳米材料表面能量转移灵敏检测水溶液中Pb2﹢的分析方法。研究表明，在Pb2﹢浓度为12.5~100 nmol/L范围内，荧光恢复效率(F/F0)与Pb2﹢浓度间呈良好的线性关系，线性回归方程为y=0.910﹢0.007c(R2=0.997)，检出限为10 nmol/L。用本方法检测自来水中Pb2﹢，结果令人满意。
형광소수식적응혈매괄배체(5′-FAM-GGT TGG TGT GGT TGG-3′)가여Pb2﹢선택성지형성G-사련체，저일구상변화능개변작위능량공체적형광염료화작위능량수체적금납미입자지간적거리，사체계적형광강도여Pb2﹢농도구유일정적상관성，종이건립료일충기우납미재료표면능량전이령민검측수용액중Pb2﹢적분석방법。연구표명，재Pb2﹢농도위12.5~100 nmol/L범위내，형광회복효솔(F/F0)여Pb2﹢농도간정량호적선성관계，선성회귀방정위y=0.910﹢0.007c(R2=0.997)，검출한위10 nmol/L。용본방법검측자래수중Pb2﹢，결과령인만의。
We established a sensitive method for the detection of lead ions in aqueous solution based on the surface energy transfer between gold nanoparticles and fluorescent dyes. The fluorescein-modified thrombin aptamer (5′-FAM-GGT TGG TGT GGT TGG-3′) can be selectively transformed to G-quadruplex induced by lead ions. The conformation changes of the aptamer can alter the distance between the energy donor of fluorescent dyes and the energy receptor of gold nanoparticles, and further enhance the fluorescence intensity. The fluorescence recovery efficiency ( F/F0 ) is proportional to the concentration of lead ions in the range of 12. 5-100 nmol/L following the linear regression equation of y=0. 910﹢0. 007c(R2=0. 997)and the limit of determination is about 10 nmol/L. The proposed method has been applied to the determination of lead ions in tap water with satisfied results.