CAJ | 학술논문

目的：探讨广西壮族自治区壮族居民HAP-2基因分布频率，指导相关血小板输注的治疗决策。方法采用聚合酶链式单核苷酸多态性方法对2659名广西壮族自治区壮族健康成年人的血液样本进行HPA-2基因分型检测，用直接测序法解决HPA-2分型差异。结果 HPA-2a/2a、HPA-2a/2b和HPA-2b/2b的等位基因频率分别为0.9157、0.0824和0.0019；测得基因多态性位点与数据库收录信息全部吻合，未发现新的单核苷酸多肽性（SNP）位点。广西壮族自治区壮族居民HPA-2a/2b基因的杂合程度与大多数亚洲国家的研究结果相似，与非洲人相比存在显著差异。结论该研究有助于确定该地区居民HPA-2多态性分布及其潜在临床意义，能更好地理解和治疗壮族免疫性介导的血小板疾病。
목적：탐토엄서장족자치구장족거민HAP-2기인분포빈솔，지도상관혈소판수주적치료결책。방법채용취합매련식단핵감산다태성방법대2659명엄서장족자치구장족건강성년인적혈액양본진행HPA-2기인분형검측，용직접측서법해결HPA-2분형차이。결과 HPA-2a/2a、HPA-2a/2b화HPA-2b/2b적등위기인빈솔분별위0.9157、0.0824화0.0019；측득기인다태성위점여수거고수록신식전부문합，미발현신적단핵감산다태성（SNP）위점。엄서장족자치구장족거민HPA-2a/2b기인적잡합정도여대다수아주국가적연구결과상사，여비주인상비존재현저차이。결론해연구유조우학정해지구거민HPA-2다태성분포급기잠재림상의의，능경호지리해화치료장족면역성개도적혈소판질병。
Objective To determine the gene distribution frequency of human platelet antigen (HPA)-2 of Guangxi Zhuang Autonomous Region,so as to guide treatment decisions concerning platelet(PLT) transfusion. Methods The polymerase chain single nucleotide polymorphism was adopted to determine the genetic typing of HPA-2 among 2 659 health adults in Guangxi Zhuang Autonomous Region,and the direct sequencing was employed to resolve the genetic difference of HPA-2. Results Allele frequencies of HPA-2a/2a,HPA-2a/2b and HPA-2b/2b were 0.915 7,0.082 4 and 0.001 9,respectively. The detected gene poly-morphism site was completely identical to the information in database ,and no new single nucleotide polymorphism (SNP) site was discov ered. The degree of heterozygosis of HPA-2a/2b of Zhuang nationality was similar to the research results of most Asian counties,but compared with the African,the difference was significant. Conclusion This study contributes to determine the polymorphism distribution of HPA-2 in Guangxi and its potential clinical significance ,and it is beneficial to understand and treat immune-mediated PLA disease of Zhuang nationality.