CAJ | 학술논문

目的：观察类风湿关节炎（ RA）患者血浆Ghrelin 水平改变及其基因C408A的多态性，进一步探讨RA发病机制。方法采集120例RA患者（ RA组）与150例健康查体者（对照组）的静脉血，采用ELISA法测定血浆Ghrelin水平，聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测Ghrelin基因C408A的多态性，分析基因型与RA患者血浆Ghrelin水平、病程、病情的关系。结果 RA组和对照组血浆Ghrelin分别为（115．03±38．12）、（156．28±65．84）pg/mL，组间比较，P＜0．05；两组基因型和等位基因频率比较均有显著差异（P均＜0．05）；RA组基因型为CC、CA、AA者血浆Ghrelin水平、病程比较均有显著差异（P均＜0．05），但病情无显著差异（P＞0．05）。结论 RA患者存在血浆Ghrelin水平降低及Ghrelin基因C408A位点变异，纯合的AA型和杂合的CA型均可比野生的CC型增加RA患病风险，但Ghrelin基因可能并非河北汉族RA的主效基因。
목적：관찰류풍습관절염（ RA）환자혈장Ghrelin 수평개변급기기인C408A적다태성，진일보탐토RA발병궤제。방법채집120례RA환자（ RA조）여150례건강사체자（대조조）적정맥혈，채용ELISA법측정혈장Ghrelin수평，취합매련반응-한제성편단장도다태성（PCR-RFLP）법검측Ghrelin기인C408A적다태성，분석기인형여RA환자혈장Ghrelin수평、병정、병정적관계。결과 RA조화대조조혈장Ghrelin분별위（115．03±38．12）、（156．28±65．84）pg/mL，조간비교，P＜0．05；량조기인형화등위기인빈솔비교균유현저차이（P균＜0．05）；RA조기인형위CC、CA、AA자혈장Ghrelin수평、병정비교균유현저차이（P균＜0．05），단병정무현저차이（P＞0．05）。결론 RA환자존재혈장Ghrelin수평강저급Ghrelin기인C408A위점변이，순합적AA형화잡합적CA형균가비야생적CC형증가RA환병풍험，단Ghrelin기인가능병비하북한족RA적주효기인。
Objective To observe the Ghrelin plasma level changes and its gene C 408A polymorphism in patients with rheumatoid arthritis ( RA) and to further investigate the pathogenesis .Methods We collected the vein blood from 120 ca-ses of RA patients ( RA group) and 150 cases of healthy people ( control group ) .The plasma level of Ghrelin was deter-mined by ELISA, and the single nucleotide polymorphisms ( SNPs) of Ghrelin gene C408A were detected by PCR-RFLP method.The relationships of genotypes with the Ghrelin plasma level , course of disease, state of disease in patients with RA were analyzed.Results The plasma levels of Ghrelin in the RA group and control group were (115.03 ±38.12) and (156.28 ±65.84) pg/mL, respectively (P<0.05); significant differences were found in the genotypes and fre-quencies between the two group (all P<0.05).The plasma level of Ghrelin and course of disease were statistically differ-ent in the genotypes of CC, CA and AA in the RA group (all P<0.05), but no significant difference was found in the state of disease (P>0.05).Conclusions In RA patients, the plasma level of Ghrelin is decreased , and there is Ghrelin gene C408A mutation.Both the homozygous AA and heterozygous CA can increase the risk of RA than the wild genotype CC, but the Ghrelin gene may be not the main gene in Hebei RA patients of the Han nationality .