CAJ | 학술논문

目的：探讨青蒿素对糖尿病大鼠肾基质金属蛋白酶-2（MMP-2）及其抑制物金属蛋白酶-2组织抑制物（ TIMP-2）表达的调节作用。方法将大鼠随机分为对照组（ A组）、糖尿病非治疗组（B组）及糖尿病青蒿素治疗组（C组），采用腹腔单剂量注射链脲佐菌素（STZ）65 mg/kg方法以建立糖尿病大鼠模型。 C组给予青蒿素300 g/（ kg· d）腹腔注射。实验进行到第3周、第6周时3组分别宰杀大鼠6只，检测肾质量/体质量、24 h尿白蛋白排泄率（UAER）及肌酐清除率（Ccr）。用免疫组化染色方法分别检测肾小球Ⅳ型胶原、TIMP-2、MMP-2和纤维连接蛋白（ FN）表达情况。结果 C组大鼠Ccr、UAER、肾质量/体质量均显著低于B组（P＜0．05或0．01）。免疫组化染色证明糖尿病大鼠肾小球Ⅳ型胶原、FN和TIMP-2表达都明显上调（P＜0．05或0．01），C组上述指标的表达都被显著抑制（P＜0．05）。 MMP-2在B组大鼠肾小球的表达显著下调（P＜0．01），C组其表达显著上调（P＜0．05）。结论青蒿素通过抑制糖尿病大鼠肾小球TIMP-2表达及增加 MMP-2表达，从而保护糖尿病实验大鼠肾脏功能。
목적：탐토청호소대당뇨병대서신기질금속단백매-2（MMP-2）급기억제물금속단백매-2조직억제물（ TIMP-2）표체적조절작용。방법장대서수궤분위대조조（ A조）、당뇨병비치료조（B조）급당뇨병청호소치료조（C조），채용복강단제량주사련뇨좌균소（STZ）65 mg/kg방법이건립당뇨병대서모형。 C조급여청호소300 g/（ kg· d）복강주사。실험진행도제3주、제6주시3조분별재살대서6지，검측신질량/체질량、24 h뇨백단백배설솔（UAER）급기항청제솔（Ccr）。용면역조화염색방법분별검측신소구Ⅳ형효원、TIMP-2、MMP-2화섬유련접단백（ FN）표체정황。결과 C조대서Ccr、UAER、신질량/체질량균현저저우B조（P＜0．05혹0．01）。면역조화염색증명당뇨병대서신소구Ⅳ형효원、FN화TIMP-2표체도명현상조（P＜0．05혹0．01），C조상술지표적표체도피현저억제（P＜0．05）。 MMP-2재B조대서신소구적표체현저하조（P＜0．01），C조기표체현저상조（P＜0．05）。결론청호소통과억제당뇨병대서신소구TIMP-2표체급증가 MMP-2표체，종이보호당뇨병실험대서신장공능。
Objective It is to explore the regular effect of artemisinin on the expression of MMP -2 and TIMP-2 in dia-betic rats.Methods The rats were randomly divided into control group ( A group), diabetic group with nontreatment ( B group),diabetic group treated with artemisinin (C group).The diabetic rat models were established by intraperitoneal injec-tion with STZ 65mg/kg.Group C was given artemisinin 300 g/( kg· d) by intraperitoneal injection .6 rats in the three groups were killed to determine kidney weight/body weight, Ccr, UAER after 3 and 6 weeks of treatment with artemisinin , mean-while FN, TIMP-2, collagen Ⅳ protein and MMP-2 expressions were measured by immunohistological staining .Results Ccr, UAER and kidney/body weight ratio in the rats of group C were significantly lower than those of the rats in group B ( P<0.05 or 0.01).Immunohistological staining showed that the protein expressions of FN , collagenⅣand TIMP-2 significantly increased in glomeruli, while MMP-2 protein significantly decreased in diabetic rats (P<0.05 or 0.01).The abnormal level of these proteins was partly reversed in group C (P<0.05).Conclusion Artemisinin has some renoprotective effects in diabet-ic rats, partly through upregulation MMP -2 and downregulation TIMP -2 activity in glomeruli .