CAJ | 학술논문

目的：观察血必净注射液对腹主动脉阻断术合并脓毒症大鼠心肌的保护作用。方法将Wistar大鼠32只随机分为对照组（ S组）、腹主动脉阻断合并脓毒症组（ I/R＋LPS组）、血必净组（ XBJ组）。 XBJ组分离腹主动脉前30 min进行血必净预处理，尾静脉注射8 mL/kg血必净注射液，腹主动脉阻断再灌注2 h后，腹腔注射LPS 20 mg/kg。所有动物于腹主动脉阻断后8 h处死，取心脏组织行HE染色观察病理损害情况，免疫组化检测各组心脏TNF-α、β2肾上腺素能受体的表达情况。结果①心脏组织病理学显示：S组未见明显的病理改变；I/R＋LPS组心肌细胞明显肿胀，肌间隙增宽，小血管充血明显，内有大量红细胞聚集，肌间隙内可见大量红细胞漏出，纤维变性、坏死，肌间隙炎性细胞明显增多聚集、贴壁，甚至出现结构模糊的溶断现象；XBJ组心肌组织紊乱，心肌细胞略肿胀，肌间隙内可见红细胞漏出、炎性细胞浸润。②免疫组织化学染色检测结果：心肌TNF-α表达数量S组＜XBJ组＜I/R＋LPS组，各组之间均有显著性差异（ P均＜0．05）；心肌β2-AR表达数量S组＞XBJ组＞I/R＋LPS组，XBJ组与I/R＋LPS及S组间比较均无显著性差异，S组与I/R＋LPS组间比较有显著性差异（P＜0．05）。结论血必净注射液预处理对腹主动脉阻断合并脓毒症大鼠早期的心肌损伤起一定的保护作用；其在缺血再灌注合并脓毒症大鼠心肌损伤的早期模型中具有下调TNF-α的作用，而对心肌β2-AR无明显影响。
목적：관찰혈필정주사액대복주동맥조단술합병농독증대서심기적보호작용。방법장Wistar대서32지수궤분위대조조（ S조）、복주동맥조단합병농독증조（ I/R＋LPS조）、혈필정조（ XBJ조）。 XBJ조분리복주동맥전30 min진행혈필정예처리，미정맥주사8 mL/kg혈필정주사액，복주동맥조단재관주2 h후，복강주사LPS 20 mg/kg。소유동물우복주동맥조단후8 h처사，취심장조직행HE염색관찰병리손해정황，면역조화검측각조심장TNF-α、β2신상선소능수체적표체정황。결과①심장조직병이학현시：S조미견명현적병리개변；I/R＋LPS조심기세포명현종창，기간극증관，소혈관충혈명현，내유대량홍세포취집，기간극내가견대량홍세포루출，섬유변성、배사，기간극염성세포명현증다취집、첩벽，심지출현결구모호적용단현상；XBJ조심기조직문란，심기세포략종창，기간극내가견홍세포루출、염성세포침윤。②면역조직화학염색검측결과：심기TNF-α표체수량S조＜XBJ조＜I/R＋LPS조，각조지간균유현저성차이（ P균＜0．05）；심기β2-AR표체수량S조＞XBJ조＞I/R＋LPS조，XBJ조여I/R＋LPS급S조간비교균무현저성차이，S조여I/R＋LPS조간비교유현저성차이（P＜0．05）。결론혈필정주사액예처리대복주동맥조단합병농독증대서조기적심기손상기일정적보호작용；기재결혈재관주합병농독증대서심기손상적조기모형중구유하조TNF-α적작용，이대심기β2-AR무명현영향。
Objective It is to observe the protecting effect of Xuebijing injection (XBJ) on cardiac muscle in the rats un-dergoing abdominal aorta clamping complicating with sepsis .Methods 32 Wistar rats were randomly divided into control group (S group), abdominal aorta clamping complicating with sepsis group (I/R+LPS group), Xuebijing injection group (XBJ group ) .The rats in XBJ group were pretreated with XBJ 30 min before abdominal aorta separated , 8 mL/kg by caudal vein in-jection, in 2 h after abdominal aorta ischemia reperfusion injury , they were given LPS20 mg/kg by intraperitoneal injection . All the animals were killed in 8 h after abdominal aorta blocking , and their cardiac tissue was gotten to do HE staining to ob-serve pathological damage , the expression of TNF -αandβ2 adrenoreceptor ( AR) in the tissue was detected by immunohisto-chemistry technique .Results①Cardiac tissue pathology results showed that: no obvious pathological change was found in S group;In I/R+LPS group myocardial cells were swelling with wider intermuscular space , little blood vessel was obviously hy-peremia with agglutination of many red blood cells which were also leaked much in the space , myocardial fibers were degenera-tion and necrosis , many inflammatory cells gathered in the space , even the structure was indistinct in cardiac tissue;In XBJ group , myocardial tissue was disorder in which the cells were slight swelling , and less leak of red blood cells and infiltration of inflammatory cells in the space was found .②Immunohistochemistry detection results showed that the expression of TNF -ɑin cardiac cells:S group<XBJ group<I/R+LPS group,the difference was significant among every group (P<0.05), the ex-pression of β2 -AR in the cells:S group>XBJ group>I/R+LPS group, the difference between S group and I/R+LPS group was significant but not in XBJ group compared with the other two groups .Conclusion XBJ pretreatment has some protecting effect on early myocardial injury in the rats undergoing abdominal aorta clamping complicating with sepsis , it can decrease the expression of TNF-αwith no obvious effect on β2 -AR in myocardial tissue in the rats .