CAJ | 학술논문

目的：探讨自然杀伤T细胞(NKT)在髋关节骨关节炎(HO)患者的表达与临床意义。方法收集45例HO患者外周血，采用流式细胞术检测其内NKT及其胞内细胞因子γ干扰素(IFN-γ)及白介素4(IL-4)的含量。并与45例类风湿性关节炎(RA)患者进行对比。结果与健康对照组比较，RA及HO组患者NKT及IFN-γ均显著降低差异有统计学意义(P<0.05)；在IL-4的比较方面，RA组显著低于对照组，但HO组IL-4与对照组比较，差异无统计学意义(P=0.098)。治疗后RA及HO组NKT、IFN-γ及IL-4均较治疗前不同程度上升，均以HO回升幅度明显，差异有统计学意义(P<0.05)，但治疗后RA组IFN-γ频率较前无统计学变化，而HO组较治疗前明显上升，且HO组IFN-γ频率明显高于RA组(P=0.006)。结论 NKT在HO免疫机制当中起到重要作用，其细胞因子IL-10在HO与RA的鉴别起到重要作用，且IFN-γ与疗效密切相关。
목적：탐토자연살상T세포(NKT)재관관절골관절염(HO)환자적표체여림상의의。방법수집45례HO환자외주혈，채용류식세포술검측기내NKT급기포내세포인자γ간우소(IFN-γ)급백개소4(IL-4)적함량。병여45례류풍습성관절염(RA)환자진행대비。결과여건강대조조비교，RA급HO조환자NKT급IFN-γ균현저강저차이유통계학의의(P<0.05)；재IL-4적비교방면，RA조현저저우대조조，단HO조IL-4여대조조비교，차이무통계학의의(P=0.098)。치료후RA급HO조NKT、IFN-γ급IL-4균교치료전불동정도상승，균이HO회승폭도명현，차이유통계학의의(P<0.05)，단치료후RA조IFN-γ빈솔교전무통계학변화，이HO조교치료전명현상승，차HO조IFN-γ빈솔명현고우RA조(P=0.006)。결론 NKT재HO면역궤제당중기도중요작용，기세포인자IL-10재HO여RA적감별기도중요작용，차IFN-γ여료효밀절상관。
Objective To explore the expression and clinical significance of natural killer T cells (NKT) in patients with hip os-teoarthritis (HO). Methods The peripheral blood of 45 patients with HO was collected. Flow cytometry was used to detect the con-tent of NKT and intracellular cytokine gamma interferon (IFN-γ) and interleukin 4 (IL-4), and compared with 45 patients with rheumatoid arthritis (RA). Results Compared with the healthy controls, NKT and IFN-γof the patients in RA group and HO group were significantly lower (P<0.05);in terms of IL-4, compared with the control group, the IL-4 of RA group was lower, but the dif-ference in IL-4 between HO group and control group was not statistically significant (P=0.098). After the treatment, the level of NKT, IFN-gamma and IL-4 of RA group and HO group increased at different levels as compared with those before treatment, and those of HO group increased significantly (P<0.05); IFN-gamma frequency of RA group after treatment showed no statistically change as compared with that before treatment, but that of HO group increased significantly compared with that before treatment, and IFN-gamma frequency of HO group was obviously higher than that of RA group (P=0.006). Conclusion The NKT play an im-portant role in the HO immunity mechanism, the cytokine IL-10 plays an important role in differentiating HO and RA, and is closely related to the curative effect of IFN-gamma.