CAJ | 학술논문

目的：寻找从业人员肠道传染病菌的快速筛查技术方法。方法以参加健康体检的从业人员作为研究对象，采集健康体检从业人员的肛拭子标本，采用荧光PCR技术批量快速筛查肠道致病菌混合样本的方法进行沙门氏菌和志贺氏菌检测，并与国家标准方法进行对照，比较两种检测方法的检出率结果。结果58176份样本中，共检出52份沙门菌阳性标本，阳性率为0.89‰，检出51份志贺氏菌阳性标本，阳性率为0.88‰。合计共检出致病菌103份，检出率为1.77‰。荧光PCR对沙门菌和志贺氏菌的灵敏度达到了100%，特异性也保持在99%以上。结论采用荧光PCR技术进行肠道致病菌的快速筛查能缩短检验时间，检验准确性高，从而有效阻断肠道传染病的传播和食物中毒的发生。
목적：심조종업인원장도전염병균적쾌속사사기술방법。방법이삼가건강체검적종업인원작위연구대상，채집건강체검종업인원적항식자표본，채용형광PCR기술비량쾌속사사장도치병균혼합양본적방법진행사문씨균화지하씨균검측，병여국가표준방법진행대조，비교량충검측방법적검출솔결과。결과58176빈양본중，공검출52빈사문균양성표본，양성솔위0.89‰，검출51빈지하씨균양성표본，양성솔위0.88‰。합계공검출치병균103빈，검출솔위1.77‰。형광PCR대사문균화지하씨균적령민도체도료100%，특이성야보지재99%이상。결론채용형광PCR기술진행장도치병균적쾌속사사능축단검험시간，검험준학성고，종이유효조단장도전염병적전파화식물중독적발생。
Objective To find out a detection method for rapidly detecting the enteric pathogens in practitioners in food industry and public places. Methods The practitioners underwent healthy examination were selected as the subjects. And the anal swab specimens of them were collected. Fluorescent PCR method for rapidly detecting batch enteric pathogens mixed samples was used to detect the Salmonella and Shigella, and was compared with the national standard method. The detection rate of these two de-tection methods was compared. Results Of the 58176 samples, 52 samples with positive Salmonella were detected, the positive rate was 0.89‰, 51 samples with positive Shigella were detected, the positive rate was 0.88‰. A total 103 pathogenic bacteria were detected, the detection rate was 1.77‰. The sensitivity and specificity of the fluorescent PCR detection method for Salmonella and Shigella was 100% and 99%, respectively. Conclusion Fluorescent PCR method for rapidly detecting the enteric bacteria can shorten the detection time but with a high diagnostic accuracy, thereby effectively blocking the spread of intestinal infectious dis-eases and the incidence of food poisoning.