现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2014年
17期
2568-2570
,共3页
锡%蛋白磷酸酶2%羊膜%抑制因子,免疫%全酶
錫%蛋白燐痠酶2%羊膜%抑製因子,免疫%全酶
석%단백린산매2%양막%억제인자,면역%전매
Tin%Protein phosphatase 2%Amnion%Suppressor factors,immunologic%Holoenzymes
目的:研究三丁基锡(TBT)对人羊膜FL细胞蛋白磷酸酶2A(PP2A)活力的抑制机制。方法 FL细胞经不同浓度TBT染毒1 h后,检测PP2A活力及PP2A组成亚基A、C、B55α和B56δ以及C亚基蛋白磷酸化和甲基化水平。结果与对照组比较,3、4、6μmol/L染毒FL细胞内PP2A活力均下降,差异有统计学意义(P<0.01);6μmol/L TBT染毒组FL细胞内PP2A组成亚基A蛋白的表达下降,4、6μmol/L TBT染毒组B55α蛋白的表达及C蛋白甲基化水平均降低,差异有统计学意义(P<0.05)。结论 TBT对FL细胞PP2A活力的抑制作用可能与其抑制B55α和A蛋白亚基表达,降低C亚基Leu309位点的甲基化水平有关。
目的:研究三丁基錫(TBT)對人羊膜FL細胞蛋白燐痠酶2A(PP2A)活力的抑製機製。方法 FL細胞經不同濃度TBT染毒1 h後,檢測PP2A活力及PP2A組成亞基A、C、B55α和B56δ以及C亞基蛋白燐痠化和甲基化水平。結果與對照組比較,3、4、6μmol/L染毒FL細胞內PP2A活力均下降,差異有統計學意義(P<0.01);6μmol/L TBT染毒組FL細胞內PP2A組成亞基A蛋白的錶達下降,4、6μmol/L TBT染毒組B55α蛋白的錶達及C蛋白甲基化水平均降低,差異有統計學意義(P<0.05)。結論 TBT對FL細胞PP2A活力的抑製作用可能與其抑製B55α和A蛋白亞基錶達,降低C亞基Leu309位點的甲基化水平有關。
목적:연구삼정기석(TBT)대인양막FL세포단백린산매2A(PP2A)활력적억제궤제。방법 FL세포경불동농도TBT염독1 h후,검측PP2A활력급PP2A조성아기A、C、B55α화B56δ이급C아기단백린산화화갑기화수평。결과여대조조비교,3、4、6μmol/L염독FL세포내PP2A활력균하강,차이유통계학의의(P<0.01);6μmol/L TBT염독조FL세포내PP2A조성아기A단백적표체하강,4、6μmol/L TBT염독조B55α단백적표체급C단백갑기화수평균강저,차이유통계학의의(P<0.05)。결론 TBT대FL세포PP2A활력적억제작용가능여기억제B55α화A단백아기표체,강저C아기Leu309위점적갑기화수평유관。
Objective To investigate the inhibition mechanism of tributyltin(TBT) on protein phosphatase 2A(PP2A) activity in human amnionic FL cells. Methods 1 h after contamination by different concentrations of TBT,FL cells were detected on the PP2A activity,levels of PP2A subunits including A,C,B55αand B56δ,protein phosphorylation of C and methylation level. Results Compared with control group,the activity of PP2A in FL cells was declined when the concentration was in 3,4 and 6μmol/L in TBT groups with statistically significant difference(P<0.01);PP2A-A subunit was decreased in 6μmol/L TBT group, and B55αsubunit and the methylation of PP2A-C were significantly decreased in the 4μmol/L and 6μmol/L TBT groups,the dif ferences all had statistically significant differnce(P<0.05). Conclusion The inhibitory effect of IBT to PP2A in FL cells may be associated with modifications of B55αand A subunit and reduction of PP2A-C methylation in Leu309 area.