山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
34期
21-23
,共3页
帕金森病%胶质细胞源性神经营养因子%多巴胺%神经元
帕金森病%膠質細胞源性神經營養因子%多巴胺%神經元
파금삼병%효질세포원성신경영양인자%다파알%신경원
glial cell line-derived neurotrophic factor%parkinsonism disease%immunohistochemistry%quantitative morphology
目的：观察向脑室或壳核内直接注射胶质细胞源性神经营养因子（ GDNF）对帕金森病大鼠黑质纹状体系统多巴胺能神经元的修复作用。方法70只大鼠，于右侧前脑内侧束（MFB）及腹侧被盖区（VTA）分别注射12μg 6-OHDA，观察2周获得39只帕金森病模型大鼠。其中15只大鼠（观察A组）脑室内注射人重组GDNF，14只（观察B组）壳核内注射人重组GDNF，5只（对照A组）脑室内注射赋形剂，5只（对照B组）壳核内注射赋形剂。采用免疫组化和量化形态学分析方法测算酪氨酸羟化酶阳性（ TH＋）神经元数目、大小及TH＋纤维密度。结果对照A、B组大鼠右侧中脑组织黑质TH＋多巴胺能神经元大小和数目及纹状体内侧部TH＋神经纤维密度均较左侧降低，P均＜0．05。观察A组与对照A组相比、观察B组与对照B组相比，双侧中脑组织黑质TH＋多巴胺能神经元大小和数目及纹状体内侧部TH＋神经纤维密度均明显升高，P均＜0．05。结论脑室或壳核内直接注射GDNF对帕金森病大鼠黑质纹状体系统多巴胺能神经元损伤有修复作用。
目的：觀察嚮腦室或殼覈內直接註射膠質細胞源性神經營養因子（ GDNF）對帕金森病大鼠黑質紋狀體繫統多巴胺能神經元的脩複作用。方法70隻大鼠，于右側前腦內側束（MFB）及腹側被蓋區（VTA）分彆註射12μg 6-OHDA，觀察2週穫得39隻帕金森病模型大鼠。其中15隻大鼠（觀察A組）腦室內註射人重組GDNF，14隻（觀察B組）殼覈內註射人重組GDNF，5隻（對照A組）腦室內註射賦形劑，5隻（對照B組）殼覈內註射賦形劑。採用免疫組化和量化形態學分析方法測算酪氨痠羥化酶暘性（ TH＋）神經元數目、大小及TH＋纖維密度。結果對照A、B組大鼠右側中腦組織黑質TH＋多巴胺能神經元大小和數目及紋狀體內側部TH＋神經纖維密度均較左側降低，P均＜0．05。觀察A組與對照A組相比、觀察B組與對照B組相比，雙側中腦組織黑質TH＋多巴胺能神經元大小和數目及紋狀體內側部TH＋神經纖維密度均明顯升高，P均＜0．05。結論腦室或殼覈內直接註射GDNF對帕金森病大鼠黑質紋狀體繫統多巴胺能神經元損傷有脩複作用。
목적：관찰향뇌실혹각핵내직접주사효질세포원성신경영양인자（ GDNF）대파금삼병대서흑질문상체계통다파알능신경원적수복작용。방법70지대서，우우측전뇌내측속（MFB）급복측피개구（VTA）분별주사12μg 6-OHDA，관찰2주획득39지파금삼병모형대서。기중15지대서（관찰A조）뇌실내주사인중조GDNF，14지（관찰B조）각핵내주사인중조GDNF，5지（대조A조）뇌실내주사부형제，5지（대조B조）각핵내주사부형제。채용면역조화화양화형태학분석방법측산락안산간화매양성（ TH＋）신경원수목、대소급TH＋섬유밀도。결과대조A、B조대서우측중뇌조직흑질TH＋다파알능신경원대소화수목급문상체내측부TH＋신경섬유밀도균교좌측강저，P균＜0．05。관찰A조여대조A조상비、관찰B조여대조B조상비，쌍측중뇌조직흑질TH＋다파알능신경원대소화수목급문상체내측부TH＋신경섬유밀도균명현승고，P균＜0．05。결론뇌실혹각핵내직접주사GDNF대파금삼병대서흑질문상체계통다파알능신경원손상유수복작용。
Objective To evaluate the restorative effect of glial cell line-derived neurotrophic factor ( GDNF) intrace-rebrallyinfusedonnigrostriataldopaminergicneuronsinratmodelwithParkinsoniandisease.Methods 70ratswereem-ployed for the modeling of Parkinsonian disease with right intracerebral( medial forebrain bundle,MFB; ventral tegmental area,VTA) infusion of 12μg 6-OHDA( 3μg /μL) ,and get 39 rat models.29 rats were received controlled intracerebral human recombined GDNF infusion (intraventricle:n=15, intraputamen:n=14) , while the other 10 rats were given ve-hicle as controls( intraventricle:n=5, intraputamenal: n=5).Immunohistochemistry and quantitative morphological a-nalysis was used to quantify the number and size of tyrosine hydroxylase positive( TH+) dopaminergic neurons and the TH+fiber density in nigrostriatal region.Results In controlled group ,the number and density of TH+dopaminergic neuron-swere in right sids increased than left.(P<0.05).Compared to controls,TH+fiber increased bilaterally in number, size and density in periventricular striatum of lesioned side.Conclusions Intracerebral GDNF infusion induces significant re-storative effect on nigrostriatal dopaminergic neurons.
