CAJ | 학술논문

目的：对原发性肝癌中医辨证分型提供客观的量化标准。方法采用微小RNA（ miRNA）芯片检测、实时定量PCR验证，对比观察原发性肝癌气虚血瘀证、肝郁血瘀证、肝胆湿热证及肝瘀痰结证患者（各3例，分别以证型命名组名）肝组织10种miRNA表达水平的差异；并与3例正常肝组织（正常组）进行比较。结果 miR-122-3p各证型组均明显高于正常组（P＜0．05），气虚血瘀组明显高于其他各证型组（P＜0．05）；miR-30b-5p在肝胆湿热组明显低于其他各证型组（P＜0．05），其他各证型组与正常组比较无显著性差异；miR-182-5p在气虚血瘀组、肝郁血瘀组和肝胆湿热组均明显高于正常组（P＜0．05）；miR-221-5p在肝胆湿热组和肝郁血瘀组明显低于其他各证型组及正常组（P＜0．05），但这两组间无显著性差异；miR-221-3p在肝郁痰结组高于其他各证型组，但无统计学差异；miR-21-5p在气虚血瘀组、肝郁血瘀组和肝郁痰结组均高于肝胆湿热组和正常组，但无统计学差异。 miR-222-3p、miR-214-3p、miR-491-3p、miR-422a各组间未见明显差异。结论原发性肝癌不同血瘀证患者肝组织miRNA水平存在显著性差异；本研究为原发性肝癌的中医辨证分型提供了客观的量化标准。
목적：대원발성간암중의변증분형제공객관적양화표준。방법채용미소RNA（ miRNA）심편검측、실시정량PCR험증，대비관찰원발성간암기허혈어증、간욱혈어증、간담습열증급간어담결증환자（각3례，분별이증형명명조명）간조직10충miRNA표체수평적차이；병여3례정상간조직（정상조）진행비교。결과 miR-122-3p각증형조균명현고우정상조（P＜0．05），기허혈어조명현고우기타각증형조（P＜0．05）；miR-30b-5p재간담습열조명현저우기타각증형조（P＜0．05），기타각증형조여정상조비교무현저성차이；miR-182-5p재기허혈어조、간욱혈어조화간담습열조균명현고우정상조（P＜0．05）；miR-221-5p재간담습열조화간욱혈어조명현저우기타각증형조급정상조（P＜0．05），단저량조간무현저성차이；miR-221-3p재간욱담결조고우기타각증형조，단무통계학차이；miR-21-5p재기허혈어조、간욱혈어조화간욱담결조균고우간담습열조화정상조，단무통계학차이。 miR-222-3p、miR-214-3p、miR-491-3p、miR-422a각조간미견명현차이。결론원발성간암불동혈어증환자간조직miRNA수평존재현저성차이；본연구위원발성간암적중의변증분형제공료객관적양화표준。
Objective To provide an objective quantitative standard for traditional Chinese medicine syndrome-types of primary hepatic carcinoma.Methods The differences about 10 kinds of miRNA expression in hepatic tissue between qi deficiency and blood stasis syndeome, liver stagnation and blood stasis syndeome, gan-dan damp-heat syndeome, liver sta-sis phlegm stagnation syndeome patients with hepatocellular carcinoma were compared by using miRNA microarray and RT PCR method.There were 3 cases in each group which named as their syndrome.All the hepatic tissues were compared with 3 cases of normal liver tissues ( normal group) .Results MiR-122-3p in hepatic tissue was higher than the normal group (P<0.05).MiR-122-3p in qi deficiency and blood stasis syndeome group was higher than the other group(P<0.05). MiR-30b-5p in gan-dan damp-heat syndeome group was lower than the other three groups(P<0.05), which was similar with the normal group.MiR-182-5p in qi deficiency and blood stasis syndeome group, liver stagnation and blood stasis syndeome group, gan-dan damp-heat syndeome group were higher than normal group(P<0.05).MiR-221-5p in liver stag-nation blood stasis syndeome group and gan-dan damp-heat syndeome group were lower than the other groups(P<0.05). But there was no significant difference between the two groups.MiR-221-3p in liver stasis phlegm stagnation syndeome group was higher than the other groups, with no statistical difference.MiR-21-5p in qi deficiency and blood stasis syndeome group, liver stagnation and blood stasis syndeome group were higher than gan-dan damp-heat syndeome group and liver stasis phlegm stagnation syndeome group, but there was no significant difference.There was no significant difference in MiR-222-3p, miR-214-3p, miR-491-3p, miR-422a of each group.Conclusions MiRNA expression in different kinds of blood stasis syndeome in primary hepatic carcinoma patients was different.This research provide an objective quantitative standard for traditional Chinese medicine syndrome differentiation of primary liver cancer classification.