CAJ | 학술논문

以尼龙膜为基质，L-色氨酸(Trp)为配基，合成了一种新的亲和介质用以吸附牛γ-球蛋白(BGG)。用批量法系统考察了温度、离子强度和pH对亲和等温吸附的影响。研究结果表明，BGG与氨基酸之间的亲和相互作用力主要是静电力和疏水相互作用力。在最适条件下吸附遵循Langmuir型吸附，并且亲和吸附量最大而非特异性吸附最小。偏离该条件则会发生蛋白质在膜上的堆积，蛋白质构型变化及蛋白质与配基间的空间取向的变化，从而使吸附不再遵循Langmuir 型吸附。
이니룡막위기질，L-색안산(Trp)위배기，합성료일충신적친화개질용이흡부우γ-구단백(BGG)。용비량법계통고찰료온도、리자강도화pH대친화등온흡부적영향。연구결과표명，BGG여안기산지간적친화상호작용력주요시정전력화소수상호작용력。재최괄조건하흡부준순Langmuir형흡부，병차친화흡부량최대이비특이성흡부최소。편리해조건칙회발생단백질재막상적퇴적，단백질구형변화급단백질여배기간적공간취향적변화，종이사흡부불재준순Langmuir 형흡부。
A new nylon affinity matrix with L-tryptoph ane as ligand was prepared for adsorption of bovine γ-globulin. Effects of tem perature, ionic strength and pH on affinity adsorption of γ-globulin on affinity membrane were studied by batch method. The results show that the interaction between ligand and γ-globulin includes mainly electrostatic and hydrophobic interaction. The affinity adsorption at optimum condition obeys Langmuir adsorption model with maximum adsorption capacity and minimum nonspecific adsorption. The space location between protein and ligand and the configuration of proteins wi ll change when deviating from this condition.