CAJ | 학술논문

目的：观察腺病毒介导的IL-10（Ad-mIL-10）基因干预对1型糖尿病（T1DM）发病早期的非肥胖型糖尿病（ NOD）鼠胰岛β细胞的保护作用。方法选取17～20周龄近期发病（诊断为T1DM不超过3 d）的雌性NOD小鼠12只，随机分为A、B组（各6只），A组发病后立即予腹腔注射0．1 mL的Ad-mIL-10，B组未予任何干预；另选取相同周龄未发病的NOD鼠6只为C组，未予任何干预。实验前及实验开始后的第1、2、3周记录小鼠体质量、尿糖及血糖；第3周时处死全部小鼠，摘眼球法取全血并测血清 IL-10、IFN-γ、IL-4和 C肽；取胰腺做病理切片，HE及免疫组化染色，观察小鼠胰岛炎性浸润程度及IL-10基因在胰腺的表达情况。结果与B组相比，A组胰腺局部IL-10高表达、血清IL-10水平升高（P＜0．01）；A组血清IFN-γ水平较B组降低、IL-4及IL-10水平较B组升高、IFN-γ／IL-4较B组降低，两组相比， P均＜0．01；A组血清C肽水平较B组升高，两组相比，P＜0．01。结论 Ad-mIL-10基因干预可对自然发病NOD小鼠发挥一定的免疫调节作用，一定程度上改善其胰岛β细胞功能。
목적：관찰선병독개도적IL-10（Ad-mIL-10）기인간예대1형당뇨병（T1DM）발병조기적비비반형당뇨병（ NOD）서이도β세포적보호작용。방법선취17～20주령근기발병（진단위T1DM불초과3 d）적자성NOD소서12지，수궤분위A、B조（각6지），A조발병후립즉여복강주사0．1 mL적Ad-mIL-10，B조미여임하간예；령선취상동주령미발병적NOD서6지위C조，미여임하간예。실험전급실험개시후적제1、2、3주기록소서체질량、뇨당급혈당；제3주시처사전부소서，적안구법취전혈병측혈청 IL-10、IFN-γ、IL-4화 C태；취이선주병리절편，HE급면역조화염색，관찰소서이도염성침윤정도급IL-10기인재이선적표체정황。결과여B조상비，A조이선국부IL-10고표체、혈청IL-10수평승고（P＜0．01）；A조혈청IFN-γ수평교B조강저、IL-4급IL-10수평교B조승고、IFN-γ／IL-4교B조강저，량조상비， P균＜0．01；A조혈청C태수평교B조승고，량조상비，P＜0．01。결론 Ad-mIL-10기인간예가대자연발병NOD소서발휘일정적면역조절작용，일정정도상개선기이도β세포공능。
Objective To obverse the protective effect on pancreas islet βcells of nonobese diabetic ( NOD) mouse in early phase of type 1 diabetes mellitus caused by adenovirus vector-mediated murine IL-10( Ad-mIL-10) genes.Methods 12 female NOD mice of 17-20 weeks old with diagnosis of type 1 diabetes mellitus less than 3 days were collected and random-ly divided into group A and B ( 6 mice in each group ) .Mice in group A were intraperitoneal injected with Ad-mIL-10 (0.1 mL) immediately in the early stage of diabetes and those in group B were treated with nothing as control .In addition, 6 NOD mice which in same age range and at inactive stage of disease were selected as group C , without any intervention .Data of weight, urine and blood sugar of mice at 4 time points which before the trial and 1, 2, 3 weeks after beginning were recor-ded for analysis .All mice were condemned to death in the third week , and whole blood was collected by method of picking eyeball, which then was used for measurement of IL-10, IFN-γ, IL-4 and C-peptide in blood serum.Several pathological sec-tions from pancreas which stained with HE and Immunohistochemistry assay were collected to analyze the infiltration degree of pancreas islet and expression of IL-10 genes in pancreas .Results Compared with group B , group A showed high IL-10 ex-pression in local pancreas and increased IL-10 figures in blood serum(P<0.01).In group A, the data of measured IFN-γand IFN-γ/IL-4 were lower than those of group B , whereas, IL-4 and IL-10 were detected in higher expression ( all P<0.01).C-peptide level in serum was more significant in group A (P<0.01).Conclusions IL-10 gene intervention has an immunomodulatory role on spontaneous disease of NOD mice .It can improve the function of islet beta cells to a certain ex-tent, but has no effect on insulitis alleviation and blood glucose control .