CAJ | 학술논문

目的：分析去白细胞悬浮红细胞、悬浮红细胞在同等离心条件下储存中的溶血率变化。方法选取2013年3月—2013年8月该站收集的90袋每袋200 mL的血液，随机分为3组，悬浮红细胞组（30袋）、去白细胞悬浮红细胞I组（30袋）、去白悬浮红细胞II组（30袋），将其3组分别放置4℃冰箱内28 d，分别7 d、14 d、21 d、28 d分别取样检测溶血率。结果悬浮红细胞组溶血率分别是7 d（0.59±0.02）、14 d（0.11±0.07）、21 d（0.13±0.08）、28 d（0.21±0.11）；去白悬红I组7 d（0.14±0.09），14 d （0.20±0.13），21 d（0.24±0.14%，28 d（0.10±0.22）；去白悬红 II 组7 d（0.10±0.06），14 d（0.21±0.10），21 d（0.32±0.16），28 d（0.41±0.18）。结论红细胞溶血与血液在去白过滤的分离中受损有关，但去白细胞悬浮红细胞在保存期溶血率小于0.8%范围内可以用于临床使用。
목적：분석거백세포현부홍세포、현부홍세포재동등리심조건하저존중적용혈솔변화。방법선취2013년3월—2013년8월해참수집적90대매대200 mL적혈액，수궤분위3조，현부홍세포조（30대）、거백세포현부홍세포I조（30대）、거백현부홍세포II조（30대），장기3조분별방치4℃빙상내28 d，분별7 d、14 d、21 d、28 d분별취양검측용혈솔。결과현부홍세포조용혈솔분별시7 d（0.59±0.02）、14 d（0.11±0.07）、21 d（0.13±0.08）、28 d（0.21±0.11）；거백현홍I조7 d（0.14±0.09），14 d （0.20±0.13），21 d（0.24±0.14%，28 d（0.10±0.22）；거백현홍 II 조7 d（0.10±0.06），14 d（0.21±0.10），21 d（0.32±0.16），28 d（0.41±0.18）。결론홍세포용혈여혈액재거백과려적분리중수손유관，단거백세포현부홍세포재보존기용혈솔소우0.8%범위내가이용우림상사용。
Objective To analyze the change of hemolysis rate of leukocyte-removed suspended red blood cells and suspended red blood cells in the storage period with the same centrifugal conditions. Methods 90 bags of 200 ml blood collected in our blood station from March 2013 to August 2013 were selected and randomly divided into 3 groups, suspended red blood cells group (30 bags), leukocyte-removed suspended red blood cells group I (30 bags), leukocyte-removed suspended red blood cells group II(30 bags). The 3 groups of bags were put in the refrigerator with the temperature of 4 centigrade for 28 days. And the three groups were sampled for detecting the hemolysis rate at the 7th day, 14th day, 21st day and 28th day. Results The hemolysis rate of sus-pended red blood cells group was 0.59%±0.02%at the 7th day, 0.11%±0.07%at the 14th day, 0.13%±0.08%at the 21st day, and 0.21%±0.11%at the 28th day;and that of the leukocyte-removed suspended red blood cells group I was 0.14%±0.09% at the 7th day, 0.20%±0.13% at the 14th day, 0.24%±0.14% at the 21st day and 0.10%±0.22% at the 28th day;and that of the leukocyte-removed suspended red blood cells group II was 0.10%±0.06% at the 7th day, 0.21%±0.10% at the 14th day, 0.32%±0.16% at the 21st day and 0.41%±0.18% at the 28th day. Conclusion The hemolysis of red blood cells is correlated to the damage due to the separation of blood for leukocyte filtration, but the leukocyte-removed suspended red blood cells can be used clinically in the storage period within the range that the hemolysis rate is below 0.8%.