CAJ | 학술논문

目的：探讨miR-137基因转染对人眼葡萄膜黑色素瘤细胞系M23细胞侵袭能力的影响及其机制。方法将正常培养的M23细胞分为转染组和对照组，每组3孔，分别采用Lipofectamine TM 2000瞬时转染miR-137 mimic及miR-137 mimics 阴性对照。转染18 h后用Transwell侵袭试验检测两组细胞侵袭能力（穿膜细胞数）；48 h后采用Western blot法检测细胞基质金属蛋白酶（ MMP）-2和MMP-9表达，采用ELISA法检测细胞培养上清液中MMP-2和MMP-9水平。结果转染组穿膜细胞数明显少于对照组（ P均＜0．05）；MMP-2和MMP-9蛋白表达低于对照组（P均＜0．05）；细胞培养上清液中MMP-2和MMP-9水平低于对照组（P均＜0．05）。结论 miR-137基因转染对M23细胞的侵袭能力有抑制作用；其机制可能为抑制细胞MMP-2和MMP-9表达。
목적：탐토miR-137기인전염대인안포도막흑색소류세포계M23세포침습능력적영향급기궤제。방법장정상배양적M23세포분위전염조화대조조，매조3공，분별채용Lipofectamine TM 2000순시전염miR-137 mimic급miR-137 mimics 음성대조。전염18 h후용Transwell침습시험검측량조세포침습능력（천막세포수）；48 h후채용Western blot법검측세포기질금속단백매（ MMP）-2화MMP-9표체，채용ELISA법검측세포배양상청액중MMP-2화MMP-9수평。결과전염조천막세포수명현소우대조조（ P균＜0．05）；MMP-2화MMP-9단백표체저우대조조（P균＜0．05）；세포배양상청액중MMP-2화MMP-9수평저우대조조（P균＜0．05）。결론 miR-137기인전염대M23세포적침습능력유억제작용；기궤제가능위억제세포MMP-2화MMP-9표체。
Objective To investigate the effects of miR-137 gene transfection on human uveal malignant melanoma cell lines M23.Methods miR-137 mimics were transfected to M23 by Lipofectamine package Lipofectamine TM 2000.Cell invasion was detected by transwell invasion assay 18 h after transfection .MMP-2 and MMP-9 proteins expression were in-vestigated by Western blot and ELISA .Results Transwell invasion assay showed that the number of M 23 cells in miR-137 mimics group was significantly lower than negative control group .Western blot and ELISA confirmed that miR-137 mimics could significantly inhibit the expressions of MMP-2/-9 protein and secreting .Conclusion miR-137 gene transfection can inhibit the invaion of human uveal malignant melanoma cell by reduce the expression of MMP -2 and MMP-9.