CAJ | 학술논문

目的：比较荧光定量分型PCR法与直接测序法检测HBV-DNA水平的应用价值。方法选取该院2012年1月-2013年12月收治的126例符合入选标准的HBV患者，分别采用荧光定量分型PCR法与直接测序法检测HBV-DNA水平，对HBV-DNA基因分型结果进行比较分析。结果126份样本只检测出3种基因分型(B型、C型、B/C混合型），其中B型占明显优势。荧光定量分型PCR法B/C混合型检出率明显高于直接测序法(P<0.001）﹔一致性检验表明两种检测方法的一致性较好(Kappa>0.75）﹔TA克隆结果与荧光定量分型PCR法一致。结论荧光定量分型PCR法检测HBV-DNA水平应用价值较直接测序法高，值得临床推广应用。
목적：비교형광정량분형PCR법여직접측서법검측HBV-DNA수평적응용개치。방법선취해원2012년1월-2013년12월수치적126례부합입선표준적HBV환자，분별채용형광정량분형PCR법여직접측서법검측HBV-DNA수평，대HBV-DNA기인분형결과진행비교분석。결과126빈양본지검측출3충기인분형(B형、C형、B/C혼합형），기중B형점명현우세。형광정량분형PCR법B/C혼합형검출솔명현고우직접측서법(P<0.001）﹔일치성검험표명량충검측방법적일치성교호(Kappa>0.75）﹔TA극륭결과여형광정량분형PCR법일치。결론형광정량분형PCR법검측HBV-DNA수평응용개치교직접측서법고，치득림상추엄응용。
Objective To compare the application value of real-time genotyping and quantitative PCR with that of direct sequenc-ing for HBV-DNA detection. Methods 126 patients with HBV admitted in our hospital and met the inclusion criteria were select-ed. The levels of HBV-DNA of the patients were detected by real-time genotyping and quantitative PCR and direct sequencing, respectively. And the genotyping results of HBV-DNA were compared and analyzed. Results 3 genotypes (B, C, B/C mixed type) were found in 126 samples, the dominant was type B. The detection rate of B/C mixed type by real-time genotyping and quantitative PCR was higher than direct sequencing (P<0.001). There was a good consistency of two methods (Kappa>0.75). TA cloning results were consistent with real-time genotyping and quantitative PCR. Conclusion The application value of real-time genotyping and quantitative PCR is higher than direct sequencing for HBV-DNA detection, so it is worthy of clinical application and promotion.