CAJ | 학술논문

目的：探讨不同离心时间及标本放置时间对凝血四项检测结果的影响。方法收集住院患者合格标本50例,按不同离心条件及放置时间进行活化部分凝血活酶时间(APTT）、凝血酶原时间(PT）、凝血酶时间(TT）、纤维蛋白原(Fib）检测。结果标本以3000 r/min离心5 min APTT、PT缩短,与离心10 min作对照差异有统计学意义(P<0.05）,TT、Fib差异无统计学意义(P>0.05）﹔标本离心8 min四项凝血指标与对照组相比差异无统计学意义(P>0.05）。标本室温放置2 h各项检测结果与即刻测定结果差异无统计学意义(P>0.05）﹔室温放置4 h、6 h APTT、PT测定结果与对照组差异有统计学意义(P<0.05）,TT、Fib差异无统计学意义(P>0.05）。结论标本3000 r/min离心8 min即可进行凝血筛选检测﹔标本放置时间对APTT、PT检测结果影响较大,标本室温放置时间不宜超过2h。
목적：탐토불동리심시간급표본방치시간대응혈사항검측결과적영향。방법수집주원환자합격표본50례,안불동리심조건급방치시간진행활화부분응혈활매시간(APTT）、응혈매원시간(PT）、응혈매시간(TT）、섬유단백원(Fib）검측。결과표본이3000 r/min리심5 min APTT、PT축단,여리심10 min작대조차이유통계학의의(P<0.05）,TT、Fib차이무통계학의의(P>0.05）﹔표본리심8 min사항응혈지표여대조조상비차이무통계학의의(P>0.05）。표본실온방치2 h각항검측결과여즉각측정결과차이무통계학의의(P>0.05）﹔실온방치4 h、6 h APTT、PT측정결과여대조조차이유통계학의의(P<0.05）,TT、Fib차이무통계학의의(P>0.05）。결론표본3000 r/min리심8 min즉가진행응혈사선검측﹔표본방치시간대APTT、PT검측결과영향교대,표본실온방치시간불의초과2h。
Objective To explore the effects of different centrifugal time and placing time of specimens on the results of 4 coagula-tion tests. Methods 50 qualified specimens of the hospitalized patients were collected, and activated partial thromboplastin time (APTT), prothrombin time(PT), thrombin time (TT), fibrinogen(Fib) were measured according to the different centrifugal conditions and placing time. Results The APTT and PT of the specimens centrifuged at 3000r/min for 5 min were shorter than those of the specimens centrifuged at the same speed for 10 min, the differences were statistically significant(P<0.05), but the differences in TT and Fib were not statistically significant(P>0.05). Comparing the 4 coagulation indexes of the specimens centrifuged for 8 min with those of the control group, the difference was not statistically significant (P>0.05). Comparing the test results of the specimens placed for 2 h at the room temperature with those of the specimens tested immediately, there was no statistically significant differ-ence(P>0.05); Comparing the test results of the specimens placed for 4 h, 6 h at the room temperature with those of the control group, the differences were statistically significant (P<0.05), but the differences in TT and Fib were not statistically significant (P>0.05). Conclusion Coagulation screening tests can be performed after the specimens are centrifuged at 3000r/min for 8 min. As the placing time can affect the test results of APTT and PT significantly, the specimens should not be placed at the room temperature more than two hours.