CAJ | 학술논문

目的：通过流式细胞仪对5／6肾切除大鼠外周血中的纤维细胞计数变化，观察外周血纤维细胞数量与肾脏纤维化程度之间的关系，探讨外周血纤维细胞作为肾纤维化早期生物学标志物的可行性。方法清洁级雄性SD大鼠10只，随机分为对照组（n＝4）和模型组（n＝6）。模型组5／6肾切除制作肾脏纤维化模型，对照组只剥离肾包膜，不切除肾实质。以CD45和Ⅰ型胶原作为循环纤维细胞的表面标记，使用流式细胞仪对两组大鼠外周血纤维细胞计数；结合肾功能生化指标及肾脏病理学改变，分析循环纤维细胞计数在肾纤维化中的意义。结果造模后第4、8、12周，模型组肾小球积分、肾小管间质积分、血清肌酐、尿素氮均高于对照组（ P均＜0．01）。模型组在造模后的第4、8、12周，外周血纤维细胞计数显著升高，高于对照组（ P均＜0．01）。外周血纤维细胞计数与血清肌酐、尿素氮呈正相关（ r分别为0．623、0．550，P均＜0．05）。结论外周血纤维细胞计数在肾纤维化早期与肾脏病理改变一致，可早期预示肾纤维化的发展进程。
목적：통과류식세포의대5／6신절제대서외주혈중적섬유세포계수변화，관찰외주혈섬유세포수량여신장섬유화정도지간적관계，탐토외주혈섬유세포작위신섬유화조기생물학표지물적가행성。방법청길급웅성SD대서10지，수궤분위대조조（n＝4）화모형조（n＝6）。모형조5／6신절제제작신장섬유화모형，대조조지박리신포막，불절제신실질。이CD45화Ⅰ형효원작위순배섬유세포적표면표기，사용류식세포의대량조대서외주혈섬유세포계수；결합신공능생화지표급신장병이학개변，분석순배섬유세포계수재신섬유화중적의의。결과조모후제4、8、12주，모형조신소구적분、신소관간질적분、혈청기항、뇨소담균고우대조조（ P균＜0．01）。모형조재조모후적제4、8、12주，외주혈섬유세포계수현저승고，고우대조조（ P균＜0．01）。외주혈섬유세포계수여혈청기항、뇨소담정정상관（ r분별위0．623、0．550，P균＜0．05）。결론외주혈섬유세포계수재신섬유화조기여신장병리개변일치，가조기예시신섬유화적발전진정。
Objective To discuss the significance of peripheral blood fibrocytes counting to renal fibrosis rats with 5/6 kidney cut .Methods CD45 and type I collagen were as the circulation fibrocytes surface markers; the flow cytometer was used to count the peripheral blood fibrocytes of rats with 5/6 kidney cut;biochemical indexes of renal function and the pathological alteration in kidney were measured .Results For the model group of rats with renal failure , in 4, 8 and 12 weeks after modeling , the peripheral blood fibrocytes counting increased significantly compared with the control group ( all P<0.01).The blood fibrocytes counting was positively correlated with renal pathology changes , Scr and BUN (P<0.05 or P<0.01).Conclusion The peripheral blood fibrocytes counting is positively correlated with renal pathology changes in early renal fibrosis , which can indicate early the development process of renal fibrosis .