CAJ | 학술논문

目的：探讨检测脓毒症大鼠血清中miR－9的表达及其意义。方法将54只大鼠分为假手术组24只、脓毒症组24只和正常对照组6只。建立盲肠结扎穿孔脓毒症动物模型。检测各组大鼠血清中白细胞介素－6（ IL－6）、肿瘤坏死因子α（ TNF－α）和C反应蛋白（ CRP）浓度水平，评估脓毒症炎症反应情况；实时荧光定量RT－PCR法检测大鼠血清中miR－9的表达情况；皮尔森相关分析评估血清miR－9的表达与IL－6、TNF－α和CRP之间的关系。结果脓毒症大鼠血清miR－9的表达水平较正常对照组和假手术组显著升高（ P均＜0．01），术后2 h血清miR－9表达即迅速上升达到一个较高水平。皮尔森相关分析结果也显示血清miR－9的表达与IL－6、TNF－α和CRP呈正相关（P均＜0．01）。结论脓毒症大鼠血清miR－9表达显著增高，其水平反映机体炎症反应状态，可以作为脓毒症早期诊断及预后的指标。
목적：탐토검측농독증대서혈청중miR－9적표체급기의의。방법장54지대서분위가수술조24지、농독증조24지화정상대조조6지。건립맹장결찰천공농독증동물모형。검측각조대서혈청중백세포개소－6（ IL－6）、종류배사인자α（ TNF－α）화C반응단백（ CRP）농도수평，평고농독증염증반응정황；실시형광정량RT－PCR법검측대서혈청중miR－9적표체정황；피이삼상관분석평고혈청miR－9적표체여IL－6、TNF－α화CRP지간적관계。결과농독증대서혈청miR－9적표체수평교정상대조조화가수술조현저승고（ P균＜0．01），술후2 h혈청miR－9표체즉신속상승체도일개교고수평。피이삼상관분석결과야현시혈청miR－9적표체여IL－6、TNF－α화CRP정정상관（P균＜0．01）。결론농독증대서혈청miR－9표체현저증고，기수평반영궤체염증반응상태，가이작위농독증조기진단급예후적지표。
Objective It is to investigates the expression of miR-9 in serum of septic rats, as well as its significance. Methods 54 rats were divided into sham group (n=24), sepsis group (n=24) and normal control group (n=6).Animal models of sepsis were established by cecal ligation and puncture.The serum levels of IL-6, TNF-αand CRP were measured in every group to evaluate the severity of sepsis.The expression level of serum miR-9 was determined by reverse transcription polymerase chain reaction ( RT-PCR) .Pearson's correlation analysis was used to evaluate if the expression of serum miR-9 had correlation with IL-6、TNF-αand CRP.Results Expression level of miR-9 in rats′serum in sepsis group was signifi-cantly higher than that in normal control group and sham group ( P<0.01) , which was very low under normal condition, was significantly increased at 2 h after CLP.Pearson's correlation analysis also showed that the level of serum miR-9 had positive correlation with IL-6、TNF-αand CRP(P<0.01).Conclusion The significantly up-regulated expression levels of serum miR-9 in septic rats reflected the situation of inflammatory response, and may be used as a diagnostic and prognostic marker in sepsis.