现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
1期
14-15,18
,共3页
张珍%魏义花%杜镇镇%周秀芝
張珍%魏義花%杜鎮鎮%週秀芝
장진%위의화%두진진%주수지
螺杆菌,幽门%监控蛋白60%肿瘤坏死因子 α%白细胞介素17%重组,遗传%U937细胞%基因%突变
螺桿菌,幽門%鑑控蛋白60%腫瘤壞死因子 α%白細胞介素17%重組,遺傳%U937細胞%基因%突變
라간균,유문%감공단백60%종류배사인자 α%백세포개소17%중조,유전%U937세포%기인%돌변
Helicobacter pylori%Chaperonin 60%Tumor necrosis factor-alpha%Interleukin-17%Recombination,genetic%U937 cells%Genes%Mutation
目的：观察幽门螺杆菌重组热休克蛋白60对U937细胞分泌TNF-α与IL-17的影响。方法将由基因定点突变、重组后表达的胃癌来源株的幽门螺杆菌重组热休克蛋白60（rhsp60，即rhsp60组）及非胃癌来源株热休克蛋白60（hsp60，即hsp60组）分别与经佛波酯（PMA）诱导分化的U937细胞共培养，调整各组蛋白最终浓度分别为50、100、200μg/mL，并在12、18、24 h时收集上清液，ELISA检测上清液中TNF-α、IL-17水平，设磷酸盐缓冲液对照组。结果rhsp60组与hsp60组TNF-α、IL-17分泌水平均明显高于对照组，且同时间点、同浓度下rhsp60组明显高于hsp60组，差异均有统计学意义（P＜0.05）。结论 rhsp60与hsp60均可促进U937细胞对细胞因子TNF-α、IL-17的分泌，且胃癌来源的rhsp60使TNF-α、IL-17分泌量更多。
目的：觀察幽門螺桿菌重組熱休剋蛋白60對U937細胞分泌TNF-α與IL-17的影響。方法將由基因定點突變、重組後錶達的胃癌來源株的幽門螺桿菌重組熱休剋蛋白60（rhsp60，即rhsp60組）及非胃癌來源株熱休剋蛋白60（hsp60，即hsp60組）分彆與經彿波酯（PMA）誘導分化的U937細胞共培養，調整各組蛋白最終濃度分彆為50、100、200μg/mL，併在12、18、24 h時收集上清液，ELISA檢測上清液中TNF-α、IL-17水平，設燐痠鹽緩遲液對照組。結果rhsp60組與hsp60組TNF-α、IL-17分泌水平均明顯高于對照組，且同時間點、同濃度下rhsp60組明顯高于hsp60組，差異均有統計學意義（P＜0.05）。結論 rhsp60與hsp60均可促進U937細胞對細胞因子TNF-α、IL-17的分泌，且胃癌來源的rhsp60使TNF-α、IL-17分泌量更多。
목적：관찰유문라간균중조열휴극단백60대U937세포분비TNF-α여IL-17적영향。방법장유기인정점돌변、중조후표체적위암래원주적유문라간균중조열휴극단백60（rhsp60，즉rhsp60조）급비위암래원주열휴극단백60（hsp60，즉hsp60조）분별여경불파지（PMA）유도분화적U937세포공배양，조정각조단백최종농도분별위50、100、200μg/mL，병재12、18、24 h시수집상청액，ELISA검측상청액중TNF-α、IL-17수평，설린산염완충액대조조。결과rhsp60조여hsp60조TNF-α、IL-17분비수평균명현고우대조조，차동시간점、동농도하rhsp60조명현고우hsp60조，차이균유통계학의의（P＜0.05）。결론 rhsp60여hsp60균가촉진U937세포대세포인자TNF-α、IL-17적분비，차위암래원적rhsp60사TNF-α、IL-17분비량경다。
Objective To investigate the effects of helicobacter pylori recombinant heat shock protein 60 on the cell se-cretion of TNF-αand IL-17 of U937 cells. Methods Hsp60 and rhsp60 were expressed by gene mutation and recombination , which were co-cultured with 50,100,200μg/mL of rhsp60(rhsp60 group) and hsp60(hsp60 group) with U937 cells induced by phorbol myristate acetate(PMA). The final concentration of the groups was adjusted as 50 μg/mL,100μg/mL,200μg/mL. The supernatant was collected at 12th,18th,24th h and IL-17,TNF-αsecretion was measured by ELISA. The experiment was setted the phosphate buffer as the control group. Results The TNF-α,IL-17 secretion concentrations in the hsp60 and rhsp60 groups were significantly higher than those in the control group. The TNF-α,IL-17 secretion concentrations in rhsp60 group was higher than that in hsp60 group at the same time and the same concentration. The difference had statistical significance (P<0.05). Conclusion The rhsp60 and hsp60 could promote the secretion of IL-17,TNF-αand rhsp60 derived from gastric cancer had more effects than hsp60 after stimulating U937 cells. secretory cell factor TNF-αand IL-17 of U937 cells.
