现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
1期
3-5,8
,共4页
潘亚萍%漆洪波%罗欣%刘西茹
潘亞萍%漆洪波%囉訢%劉西茹
반아평%칠홍파%라흔%류서여
补体因子D%脂联素%子痫%脂肪因子类%胎盘%血清%尿%孕妇
補體因子D%脂聯素%子癇%脂肪因子類%胎盤%血清%尿%孕婦
보체인자D%지련소%자간%지방인자류%태반%혈청%뇨%잉부
Complement factor D%Adiponectin%Eclampsia%Adipokines%Placenta%Serum%Urine%Pregnant women
目的：检测子痫前期孕妇胎盘组织、血清及尿液中补体因子D（CFD）以及脂联素（ADPN）的水平，分析二者的相关性及其临床意义。方法选取2014年3~9月在重庆医科大学附属第一医院产科住院行剖宫产术的子痫前期孕妇29例（子痫前期组）和正常足月妊娠行择期剖宫产术的孕妇30例（正常妊娠组），采用Western blotting检测正常妊娠组和子痫前期组孕妇胎盘组织中CFD、ADPN的表达水平。采用ELISA方法检测两组孕妇血清及尿液中CFD、ADPN水平，计算2个指标的组间水平差异，并分析二者的相关性。结果（1）胎盘组织中，与正常妊娠组比较，子痫前期组孕妇CFD表达水平显著升高，而ADPN水平显著下降，差异均有统计学意义（P<0.05或0.01）。（2）正常妊娠组孕妇血清及尿液中CFD水平为（0.67±0.04）μg/mL、（43.27±16.59）ng/mL，显著低于子痫前期组[（1.83±0.59）μg/mL、（174.95±23.61）ng/mL]，差异均有统计学意义（P<0.01）；正常妊娠组孕妇血清及尿液中ADPN水平[（80.68±1.27）、（9.29±1.33）ng/mL]显著高于子痫前期组[（13.16±2.01）、（2.40±0.86）ng/mL]，差异均有统计学意义（P<0.05）。（3）在子痫前期组孕妇的胎盘组织、血清及尿液中，CFD、ADPN水平均呈显著负相关（r=-0.82、-0.78、-0.63，P<0.05）。结论子痫前期孕妇胎盘、血清和尿液中CFD、ADPN水平的异常变化与子痫前期发病有关，二者可能存在协同作用。联合检测孕妇血清和尿液中CFD、ADPN水平有望成为预测及诊断子痫前期的一种新手段。
目的：檢測子癇前期孕婦胎盤組織、血清及尿液中補體因子D（CFD）以及脂聯素（ADPN）的水平，分析二者的相關性及其臨床意義。方法選取2014年3~9月在重慶醫科大學附屬第一醫院產科住院行剖宮產術的子癇前期孕婦29例（子癇前期組）和正常足月妊娠行擇期剖宮產術的孕婦30例（正常妊娠組），採用Western blotting檢測正常妊娠組和子癇前期組孕婦胎盤組織中CFD、ADPN的錶達水平。採用ELISA方法檢測兩組孕婦血清及尿液中CFD、ADPN水平，計算2箇指標的組間水平差異，併分析二者的相關性。結果（1）胎盤組織中，與正常妊娠組比較，子癇前期組孕婦CFD錶達水平顯著升高，而ADPN水平顯著下降，差異均有統計學意義（P<0.05或0.01）。（2）正常妊娠組孕婦血清及尿液中CFD水平為（0.67±0.04）μg/mL、（43.27±16.59）ng/mL，顯著低于子癇前期組[（1.83±0.59）μg/mL、（174.95±23.61）ng/mL]，差異均有統計學意義（P<0.01）；正常妊娠組孕婦血清及尿液中ADPN水平[（80.68±1.27）、（9.29±1.33）ng/mL]顯著高于子癇前期組[（13.16±2.01）、（2.40±0.86）ng/mL]，差異均有統計學意義（P<0.05）。（3）在子癇前期組孕婦的胎盤組織、血清及尿液中，CFD、ADPN水平均呈顯著負相關（r=-0.82、-0.78、-0.63，P<0.05）。結論子癇前期孕婦胎盤、血清和尿液中CFD、ADPN水平的異常變化與子癇前期髮病有關，二者可能存在協同作用。聯閤檢測孕婦血清和尿液中CFD、ADPN水平有望成為預測及診斷子癇前期的一種新手段。
목적：검측자간전기잉부태반조직、혈청급뇨액중보체인자D（CFD）이급지련소（ADPN）적수평，분석이자적상관성급기림상의의。방법선취2014년3~9월재중경의과대학부속제일의원산과주원행부궁산술적자간전기잉부29례（자간전기조）화정상족월임신행택기부궁산술적잉부30례（정상임신조），채용Western blotting검측정상임신조화자간전기조잉부태반조직중CFD、ADPN적표체수평。채용ELISA방법검측량조잉부혈청급뇨액중CFD、ADPN수평，계산2개지표적조간수평차이，병분석이자적상관성。결과（1）태반조직중，여정상임신조비교，자간전기조잉부CFD표체수평현저승고，이ADPN수평현저하강，차이균유통계학의의（P<0.05혹0.01）。（2）정상임신조잉부혈청급뇨액중CFD수평위（0.67±0.04）μg/mL、（43.27±16.59）ng/mL，현저저우자간전기조[（1.83±0.59）μg/mL、（174.95±23.61）ng/mL]，차이균유통계학의의（P<0.01）；정상임신조잉부혈청급뇨액중ADPN수평[（80.68±1.27）、（9.29±1.33）ng/mL]현저고우자간전기조[（13.16±2.01）、（2.40±0.86）ng/mL]，차이균유통계학의의（P<0.05）。（3）재자간전기조잉부적태반조직、혈청급뇨액중，CFD、ADPN수평균정현저부상관（r=-0.82、-0.78、-0.63，P<0.05）。결론자간전기잉부태반、혈청화뇨액중CFD、ADPN수평적이상변화여자간전기발병유관，이자가능존재협동작용。연합검측잉부혈청화뇨액중CFD、ADPN수평유망성위예측급진단자간전기적일충신수단。
Objective To detect the expression and correlation of complement factor D (CFD) and adiponectin (ADPN) in the placenta tissues,serum and urine in pregnancy with preeclampsia. Methods It was selected 29 pregnant women with preeclampsia undergoing cesarean section (the preeclampsia group) and 30 normal full-term pregnant women with elective cae sarean section(the normal pregnancy group) admitted by the Department of Obstetrics of the First Affiliated Hospital of Chongqing Medical University from March to September in 2014. It was adopted Western blotting to detect the CFD and ADPN concentra-tions in placenta tissues and ELISA to detect the CFD and ADPN concentrations in serum and urine , calculating the two indicators′difference between the two groups and analyzing the correlation of the two. Results (1)The expression concentration of CFD protein of preeclamp sia patients was significantly increased in the placenta tissues compared to the normal pregnancy group(P<0.05 or 0.01). (2)The serum and urine concentrations of CFD in the normal pregnancy group were significantly lower than those in the preeclampsia group[(0.67±0.04)μg/mL,(43.27±16.59)ng/mL vs(1.83±0.59)μg/mL,(174.95±23.61)ng/mL, P<0.01];The serum and urine concentrations of ADPN in the normal pregnancy group were significantly higher than those in the preeclampsia group[(80.68±1.27),(9.29±1.33)ng/mL vs(13.16±2.01),(2.40±0.86)ng/mL,P<0.01]. (3) It showed a significantly negative correlation of CFD and ADPN concentrations in the placenta tissues ,serum and urine in pregnancy with preeclampsia (r=-0.82,-0.78,-0.63,P<0.05). Conclusion The abnormal changes of CFD and ADPN concentrations in the placental tissues , serum and urine in pregnant women may contribute to the pathology of preeclampsia ,and the two indicators may have synergistic effects. Taking advantage of combined detection of the CFD and ADPN concentrations in maternal serum or urine may be a promising method for prediction and diagnosis of preeclampsia.
