山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
8期
7-10
,共4页
陈洪涛%刘源焕%覃学谦%蔡丹昭
陳洪濤%劉源煥%覃學謙%蔡丹昭
진홍도%류원환%담학겸%채단소
广山楂总黄酮%大孔吸附树脂%分离纯化
廣山楂總黃酮%大孔吸附樹脂%分離純化
엄산사총황동%대공흡부수지%분리순화
total flavones of Malus doumeri ( Bois) Chev%macroreticular resin%separation and purification
目的：探讨大孔吸附树脂分离纯化广山楂总黄酮的最佳工艺条件及参数。方法以静态和动态两个方面的饱和吸附量及洗脱率为指标，比较非极性树脂D101、弱极性树脂AB-8、中极性树脂HPD-450用于广山楂总黄酮分离纯化的优劣，并通过考察总黄酮回收率，优化所选最佳树脂的吸附工艺参数。结果 D101、AB-8、HPD-450静态饱和吸附量分别为94．802、118．494、98．317 mg／g干树脂，静态洗脱率分别为83．434％、91．253％、86．831％，洗脱液总黄酮量分别为91．490、115．567、98．528 mg，总黄酮洗脱率分别为85．852％、93．217％、89．051％。以5倍树脂体积的70％乙醇洗脱，流速1．5 mL／min，所得总黄酮回收率为94．09％，纯度为73．77％。结论 AB-8型树脂综合性能最好，其最佳吸附工艺参数为以5倍树脂体积的70％乙醇洗脱，流速1．5 mL／min。
目的：探討大孔吸附樹脂分離純化廣山楂總黃酮的最佳工藝條件及參數。方法以靜態和動態兩箇方麵的飽和吸附量及洗脫率為指標，比較非極性樹脂D101、弱極性樹脂AB-8、中極性樹脂HPD-450用于廣山楂總黃酮分離純化的優劣，併通過攷察總黃酮迴收率，優化所選最佳樹脂的吸附工藝參數。結果 D101、AB-8、HPD-450靜態飽和吸附量分彆為94．802、118．494、98．317 mg／g榦樹脂，靜態洗脫率分彆為83．434％、91．253％、86．831％，洗脫液總黃酮量分彆為91．490、115．567、98．528 mg，總黃酮洗脫率分彆為85．852％、93．217％、89．051％。以5倍樹脂體積的70％乙醇洗脫，流速1．5 mL／min，所得總黃酮迴收率為94．09％，純度為73．77％。結論 AB-8型樹脂綜閤性能最好，其最佳吸附工藝參數為以5倍樹脂體積的70％乙醇洗脫，流速1．5 mL／min。
목적：탐토대공흡부수지분리순화엄산사총황동적최가공예조건급삼수。방법이정태화동태량개방면적포화흡부량급세탈솔위지표，비교비겁성수지D101、약겁성수지AB-8、중겁성수지HPD-450용우엄산사총황동분리순화적우렬，병통과고찰총황동회수솔，우화소선최가수지적흡부공예삼수。결과 D101、AB-8、HPD-450정태포화흡부량분별위94．802、118．494、98．317 mg／g간수지，정태세탈솔분별위83．434％、91．253％、86．831％，세탈액총황동량분별위91．490、115．567、98．528 mg，총황동세탈솔분별위85．852％、93．217％、89．051％。이5배수지체적적70％을순세탈，류속1．5 mL／min，소득총황동회수솔위94．09％，순도위73．77％。결론 AB-8형수지종합성능최호，기최가흡부공예삼수위이5배수지체적적70％을순세탈，류속1．5 mL／min。
Objective To explore the optimum technological parameters of the separation and purification process of total flavones from Malus doumeri (Bois) Chev with macroreticular resins.Methods With the static and dynamic saturat-ed adsorption and elution rate as index , compare the advantages of nonpolar resin D 101 , weak polarity resin AB-8, medium polarity resin HPD-450 used in isolation and purification of total flavones from Malus doumeri (Bois) Chev, and optimize the adsorption process parameters of selected optimum resin by investigating the total flavones recovery rate .Results The static saturated adsorption of D 101 , AB-8, HPD-450 were 94.802, 118.494, 98.317 mg/g dry resin, the static elution rate were 83.434%, 91.253%and 83.434%respectively;the amount of total flavones eluted were 91.490, 115.567, 98.528 mg, elution rate were 85.852%, 93.217%and 89.051%respectively.Eluted with five times resin volume of 70%etha-nol, flow rate of 1.5 mL/min, the recovery rate of total flavones was 94.09%, the purity of which was 73.77%.Conclu-sion The comprehensive performance of AB-8 resin was the best , the optimum process parameters were that of eluted with five times resin volume of 70%ethanol and 1.5 mL/min flow rate.
