CAJ | 학술논문

目的：探讨P63／平滑肌肌动蛋白（ SMA）双标记法免疫组化检查对乳腺癌诊断的效能。方法体检发现乳腺肿块的患者150例，行乳腺针吸检查取病变组织，分别进行HE染色和P63／SMA双标记法免疫组化染色，光镜下观察结果并分析。结果乳腺肌上皮细胞同时表达P63和SMA，非肌上皮细胞均不表达P63。 HE染色、P63／SMA双标记法免疫组化染色检查分别有32例、20例初诊报告为乳腺良性病变，分别有119例、121例初诊报告为可疑癌细胞或发现癌细胞。对比患者手术病理结果，HE染色对乳腺良性病变的诊断准确率为91％，P63／SMA双标记法免疫组化检查为97％；HE染色和P63／SMA双标记法免疫组化检查对乳腺恶性病变的诊断准确率均为100％。结论 P63／SMA双标记法免疫组化检查对乳腺癌的确诊率较高，且较HE染色可更好地鉴别乳腺良性病变。
목적：탐토P63／평활기기동단백（ SMA）쌍표기법면역조화검사대유선암진단적효능。방법체검발현유선종괴적환자150례，행유선침흡검사취병변조직，분별진행HE염색화P63／SMA쌍표기법면역조화염색，광경하관찰결과병분석。결과유선기상피세포동시표체P63화SMA，비기상피세포균불표체P63。 HE염색、P63／SMA쌍표기법면역조화염색검사분별유32례、20례초진보고위유선량성병변，분별유119례、121례초진보고위가의암세포혹발현암세포。대비환자수술병리결과，HE염색대유선량성병변적진단준학솔위91％，P63／SMA쌍표기법면역조화검사위97％；HE염색화P63／SMA쌍표기법면역조화검사대유선악성병변적진단준학솔균위100％。결론 P63／SMA쌍표기법면역조화검사대유선암적학진솔교고，차교HE염색가경호지감별유선량성병변。
Objective To investigate the diagnostic efficiency of P63/smooth muscle actin ( SMA) double labeled im-munochemistry in lesion tissues of breast cancer.Methods A total of 150 cases of patients with breast disease were select-ed as the subjects.Needle aspiration was performed to attain the breast specimens.HE staining and double labeled immu-nohistochemical staining of P63 and SMA were used.The results were observed and analyzed by using light microscope. Results Both P63 and SMA were positive in the vast majority of breast myoepithelial cells and P63 was negative in non-myoepithelial cells.Thirty-two cases by HE staining and 20 cases by double labeled immunohistochemical staining were di-agnosed with benign breast lesions, respectively;and 119 cases by HE staining and 121 cases by double labeled immuno-histochemical staining of suspicious carcinoma cells or carcinoma cell were found.When we compared the pathological re-sults, the accuracy rates in diagnosis of benign breast lesions with HE staining and double labeled immunohistochemical staining were 91%and 97%, respectively.The diagnostic accuracy rates of malignant breast lesions were 100% by both HE and double labeled immunohistochemical staining.Conclusion P63 and SMA double labeled immunohistochemical staining has higher accuracy rate in diagnosis of breast cancer and it could effectively diagnose the benign breast lesions.