山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
15期
4-7
,共4页
王永强%金黑鹰%李丽梅%王水明%吴闯
王永彊%金黑鷹%李麗梅%王水明%吳闖
왕영강%금흑응%리려매%왕수명%오틈
冬凌草甲素%冬凌草甲素纳米结晶%人结肠癌细胞%细胞增殖%细胞凋亡%细胞周期%体外试验
鼕凌草甲素%鼕凌草甲素納米結晶%人結腸癌細胞%細胞增殖%細胞凋亡%細胞週期%體外試驗
동릉초갑소%동릉초갑소납미결정%인결장암세포%세포증식%세포조망%세포주기%체외시험
oridonin%nano-crystalline of oridonin%human colorectal cancer cells%cell proliferation%apoptosis%cell cycle%in vitro experiment
目的：制备冬凌草甲素纳米结晶，并观察其在体外对结肠癌细胞增殖及凋亡的影响。方法采用高压均质法制备冬凌草甲素纳米混悬液，加入5％甘露醇振摇溶解后冻干获得冬凌草甲素纳米结晶，测定体外溶出度。取四种人结肠癌细胞株（ Lovo细胞、SW480细胞、HCT-116细胞、HCT-8细胞），分别加入0、5、10、15、25μg／mL的冬凌草甲素纳米结晶进行培养，细胞计数法观察细胞增殖抑制情况。分别加入5、10、25μg／mL的冬凌草甲素纳米结晶培养上述结肠癌细胞，观察细胞凋亡情况及细胞周期。结果成功制备冬凌草甲素纳米混悬液及纳米结晶。加入不同浓度冬凌草甲素纳米结晶24 h后结肠癌细胞增殖抑制率与加药72 h后相比，P均＜0．05；其中25μg／mL的冬凌草甲素纳米结晶对结肠癌细胞增殖抑制及促凋亡作用最强（P均＜0．05）。冬凌草甲素纳米结晶能将Lovo细胞、HCT-8细胞的细胞周期阻滞在G1期，并将HCT-116细胞、SW480细胞的细胞周期阻滞在S期。结论制备的冬凌草甲素纳米结晶体外溶出度更高，其对体外培养的四种结肠癌细胞株均有明显的增殖抑制作用，并能诱导结肠癌细胞凋亡，阻滞细胞周期。
目的：製備鼕凌草甲素納米結晶，併觀察其在體外對結腸癌細胞增殖及凋亡的影響。方法採用高壓均質法製備鼕凌草甲素納米混懸液，加入5％甘露醇振搖溶解後凍榦穫得鼕凌草甲素納米結晶，測定體外溶齣度。取四種人結腸癌細胞株（ Lovo細胞、SW480細胞、HCT-116細胞、HCT-8細胞），分彆加入0、5、10、15、25μg／mL的鼕凌草甲素納米結晶進行培養，細胞計數法觀察細胞增殖抑製情況。分彆加入5、10、25μg／mL的鼕凌草甲素納米結晶培養上述結腸癌細胞，觀察細胞凋亡情況及細胞週期。結果成功製備鼕凌草甲素納米混懸液及納米結晶。加入不同濃度鼕凌草甲素納米結晶24 h後結腸癌細胞增殖抑製率與加藥72 h後相比，P均＜0．05；其中25μg／mL的鼕凌草甲素納米結晶對結腸癌細胞增殖抑製及促凋亡作用最彊（P均＜0．05）。鼕凌草甲素納米結晶能將Lovo細胞、HCT-8細胞的細胞週期阻滯在G1期，併將HCT-116細胞、SW480細胞的細胞週期阻滯在S期。結論製備的鼕凌草甲素納米結晶體外溶齣度更高，其對體外培養的四種結腸癌細胞株均有明顯的增殖抑製作用，併能誘導結腸癌細胞凋亡，阻滯細胞週期。
목적：제비동릉초갑소납미결정，병관찰기재체외대결장암세포증식급조망적영향。방법채용고압균질법제비동릉초갑소납미혼현액，가입5％감로순진요용해후동간획득동릉초갑소납미결정，측정체외용출도。취사충인결장암세포주（ Lovo세포、SW480세포、HCT-116세포、HCT-8세포），분별가입0、5、10、15、25μg／mL적동릉초갑소납미결정진행배양，세포계수법관찰세포증식억제정황。분별가입5、10、25μg／mL적동릉초갑소납미결정배양상술결장암세포，관찰세포조망정황급세포주기。결과성공제비동릉초갑소납미혼현액급납미결정。가입불동농도동릉초갑소납미결정24 h후결장암세포증식억제솔여가약72 h후상비，P균＜0．05；기중25μg／mL적동릉초갑소납미결정대결장암세포증식억제급촉조망작용최강（P균＜0．05）。동릉초갑소납미결정능장Lovo세포、HCT-8세포적세포주기조체재G1기，병장HCT-116세포、SW480세포적세포주기조체재S기。결론제비적동릉초갑소납미결정체외용출도경고，기대체외배양적사충결장암세포주균유명현적증식억제작용，병능유도결장암세포조망，조체세포주기。
Objective To prepare the nano-crystalline of oridonin and to investigate the effect on the proliferation and apoptosis of colorectal cancer cells.Methods The contents of oridonin nanosuspensions were prepared by high pressure homogenization.The nano-crystalline of oridonin was obtained after adding 5% mannitol, and then shaking and lyophili-zing.The solubility was determined in vitro.Colorectal cancer cells ( Lovo cells, SW480 cells, HCT-116 cells and HCT-8 cells) were cultured in vitro and incubated with different concentrations of nano-crystalline of oridonin (0, 5, 10, 15 and 25 μg/mL) .Cell proliferation was detected by cytometry.The apoptosis and cell cycle of cultured cells in different concen-trations of nano-crystalline of oridonin (5, 10 and 25 μg/mL) were detected.Results The nanosuspensions and nano-crystalline of oridonin was prepared successfully.There were significant differences in the proliferation inhibition rate among four kinds of colorectal cancer cells that were intervened by different concentrations of nano-crystalline of oridonin for 24 h and 72 h ( all P<0.05 ) .The nano-crystalline of oridonin with concentration of 25 μg/mL had the strongest inhibitory effect on cell proliferation as compared with the others (all P<0.05).The nano-crystalline of oridonin could block cell cy-cle of the Lovo cells and HCT-8 cells at G1 phase, and block the HCT-16 and SW480 cell cycle at S phase.Conclusion The vitro dissolution of nano-crystalline of oridonin is higher than oridonin.The nano-crystalline of oridonin can significant-ly inhibit the proliferation, induce the apoptosis and block the cell cycle of four kinds of colorectal cancer cells.
