现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
16期
1734-1737
,共4页
王鑫%赵艳霞%李海霞%李爱军
王鑫%趙豔霞%李海霞%李愛軍
왕흠%조염하%리해하%리애군
百草枯%中毒%Caspase-8%Caspase-9%肾损伤%细胞凋亡
百草枯%中毒%Caspase-8%Caspase-9%腎損傷%細胞凋亡
백초고%중독%Caspase-8%Caspase-9%신손상%세포조망
paraquat%poisoning%Caspase-8%Caspase-9%renal injury%cell apoptosis
目的：观察Caspase－8、Caspase－9在急性百草枯中毒大鼠肾组织中的表达情况，并探讨二者在百草枯中毒肾损伤中所起的作用。方法将140只SD大鼠（雌雄各半）随机分成对照组70只、染毒组70只，对照组给予生理盐水灌胃，染毒组给予50 mg／kg百草枯灌胃染毒。2组分别于灌胃后1，3，7，14，21，28，35d取肾组织标本行光镜下观察肾组织病理变化，用免疫组化染色法测定Caspase －8、Caspase －9的表达情况，原位末端标记法（ TUNEL法）标记凋亡细胞。结果染毒组大鼠肾组织中Caspase－8从第1天就明显表达，第3天达到高峰，第35天虽有下降，但仍高于对照组；而Caspase－9的表达第1天明显增加，第14天达到高峰，第35天仍明显高于对照组。染毒组第3天凋亡指数增加，第21天达到高峰，第35天仍高于对照组。结论 Caspase－8、Caspase－9在百草枯中毒大鼠肾组织的表达发生改变，细胞凋亡是百草枯导致肾损伤的机制之一。
目的：觀察Caspase－8、Caspase－9在急性百草枯中毒大鼠腎組織中的錶達情況，併探討二者在百草枯中毒腎損傷中所起的作用。方法將140隻SD大鼠（雌雄各半）隨機分成對照組70隻、染毒組70隻，對照組給予生理鹽水灌胃，染毒組給予50 mg／kg百草枯灌胃染毒。2組分彆于灌胃後1，3，7，14，21，28，35d取腎組織標本行光鏡下觀察腎組織病理變化，用免疫組化染色法測定Caspase －8、Caspase －9的錶達情況，原位末耑標記法（ TUNEL法）標記凋亡細胞。結果染毒組大鼠腎組織中Caspase－8從第1天就明顯錶達，第3天達到高峰，第35天雖有下降，但仍高于對照組；而Caspase－9的錶達第1天明顯增加，第14天達到高峰，第35天仍明顯高于對照組。染毒組第3天凋亡指數增加，第21天達到高峰，第35天仍高于對照組。結論 Caspase－8、Caspase－9在百草枯中毒大鼠腎組織的錶達髮生改變，細胞凋亡是百草枯導緻腎損傷的機製之一。
목적：관찰Caspase－8、Caspase－9재급성백초고중독대서신조직중적표체정황，병탐토이자재백초고중독신손상중소기적작용。방법장140지SD대서（자웅각반）수궤분성대조조70지、염독조70지，대조조급여생리염수관위，염독조급여50 mg／kg백초고관위염독。2조분별우관위후1，3，7，14，21，28，35d취신조직표본행광경하관찰신조직병리변화，용면역조화염색법측정Caspase －8、Caspase －9적표체정황，원위말단표기법（ TUNEL법）표기조망세포。결과염독조대서신조직중Caspase－8종제1천취명현표체，제3천체도고봉，제35천수유하강，단잉고우대조조；이Caspase－9적표체제1천명현증가，제14천체도고봉，제35천잉명현고우대조조。염독조제3천조망지수증가，제21천체도고봉，제35천잉고우대조조。결론 Caspase－8、Caspase－9재백초고중독대서신조직적표체발생개변，세포조망시백초고도치신손상적궤제지일。
Objective It is to investigate the expressions of Caspase -8, Caspase-9 in the renal tissue of acute paraquat-induced poisoning rats , and to explore their function in renal injury induced by paraquat poison .Methods One hundred and forty healthy adult Sprague -Dawley ( SD) rats with half males and half females were randomly divided into tow groups:con-trol group with 70 rats which were given normal saline by gavage and poisoned group with 70 rats which were given 50 mg/kg paraquat by gavage .At 1,3, 7, 14, 21, 28 and 35 days after poisoning , examples of renal tissue of the rats in both groups were gotten to observe the pathological changes of renal tissue under light microscope , and the expression of Caspase -8, Caspase-9 in kidney were evaluated by immunohistochemistry .TUNEL was used to label apoptotic cells .Results Compared with control group , the expression of Caspase -8 in renal tissue of poisoned group significantly rose at the first day , reached the peak at the 3th day and afterwards decreased slowly , at 35th day, the expression was still higher than that in the control group . The expression of Caspase -9 reached the peak at the 14th day, and kept at relatively high levels up to the 35th day.Apoptosis index of poisoned group significantly rose at the thrid day , reached the peak at the 21th day and at 35th day, the index was still higher than that in the control group .than those in group A .Conclusion The expressions of Caspase -8, Caspase -9 were changed in renal injury of acute paraquat-poisoning rats.The cell apoptosis is one of the mechanism leading to kidney damage .
