现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
16期
1724-1726
,共3页
刘晓军%陈重%李多云%程航%邓向斌%邓名贵%潘伟光%杨唯枝%王红燕%姚伟明%余治健%邓启文
劉曉軍%陳重%李多雲%程航%鄧嚮斌%鄧名貴%潘偉光%楊唯枝%王紅燕%姚偉明%餘治健%鄧啟文
류효군%진중%리다운%정항%산향빈%산명귀%반위광%양유지%왕홍연%요위명%여치건%산계문
金黄色葡萄球菌%利奈唑胺%耐药5%0S核糖体蛋白
金黃色葡萄毬菌%利奈唑胺%耐藥5%0S覈糖體蛋白
금황색포도구균%리내서알%내약5%0S핵당체단백
Staphylococcus aureus%Linezolid resistance%50S ribosomal protein
目的：阐明体外诱导利奈唑胺耐药金黄色葡萄球菌的核糖体蛋白位点变异特征。方法收集2株金黄色葡萄球菌质控菌ATCC29213和ATCC25923、1株乳汁来源的金黄色葡萄球菌和1株血流感染的金黄色葡萄球菌（编号分别为S2、S3、S5和S7菌株，均为甲氧西林和利奈唑胺敏感株），通过体外浓度倍增法诱导利奈唑胺耐药；挑取单克隆，经E－test条测定MIC值，获得各菌株的耐药浓度梯度；提取耐药菌株基因组DNA，PCR扩增核糖体蛋白L3和L4（对应rplC和rplD基因），扩增产物经测序后与野生株比较，获得核糖体蛋白对应氨基酸的突变位点。结果经体外多步法诱导利奈唑胺耐药的不同MIC值金黄色葡萄球菌共24株。 PCR测序分析4株母株均无变异位点，各菌株L3对应的氨基酸位点不尽相同，S2组菌株L4未见统一的氨基酸位点，余S3、S5、S7各组菌株普遍存在Ala56Asp 氨基酸位点变异。结论体外多步法可诱导金黄色葡萄球菌利奈唑胺耐药，耐药机制与核糖体蛋白位点突变密切相关，但仍需进一步研究证实突变位点与耐药的关系。
目的：闡明體外誘導利奈唑胺耐藥金黃色葡萄毬菌的覈糖體蛋白位點變異特徵。方法收集2株金黃色葡萄毬菌質控菌ATCC29213和ATCC25923、1株乳汁來源的金黃色葡萄毬菌和1株血流感染的金黃色葡萄毬菌（編號分彆為S2、S3、S5和S7菌株，均為甲氧西林和利奈唑胺敏感株），通過體外濃度倍增法誘導利奈唑胺耐藥；挑取單剋隆，經E－test條測定MIC值，穫得各菌株的耐藥濃度梯度；提取耐藥菌株基因組DNA，PCR擴增覈糖體蛋白L3和L4（對應rplC和rplD基因），擴增產物經測序後與野生株比較，穫得覈糖體蛋白對應氨基痠的突變位點。結果經體外多步法誘導利奈唑胺耐藥的不同MIC值金黃色葡萄毬菌共24株。 PCR測序分析4株母株均無變異位點，各菌株L3對應的氨基痠位點不儘相同，S2組菌株L4未見統一的氨基痠位點，餘S3、S5、S7各組菌株普遍存在Ala56Asp 氨基痠位點變異。結論體外多步法可誘導金黃色葡萄毬菌利奈唑胺耐藥，耐藥機製與覈糖體蛋白位點突變密切相關，但仍需進一步研究證實突變位點與耐藥的關繫。
목적：천명체외유도리내서알내약금황색포도구균적핵당체단백위점변이특정。방법수집2주금황색포도구균질공균ATCC29213화ATCC25923、1주유즙래원적금황색포도구균화1주혈류감염적금황색포도구균（편호분별위S2、S3、S5화S7균주，균위갑양서림화리내서알민감주），통과체외농도배증법유도리내서알내약；도취단극륭，경E－test조측정MIC치，획득각균주적내약농도제도；제취내약균주기인조DNA，PCR확증핵당체단백L3화L4（대응rplC화rplD기인），확증산물경측서후여야생주비교，획득핵당체단백대응안기산적돌변위점。결과경체외다보법유도리내서알내약적불동MIC치금황색포도구균공24주。 PCR측서분석4주모주균무변이위점，각균주L3대응적안기산위점불진상동，S2조균주L4미견통일적안기산위점，여S3、S5、S7각조균주보편존재Ala56Asp 안기산위점변이。결론체외다보법가유도금황색포도구균리내서알내약，내약궤제여핵당체단백위점돌변밀절상관，단잉수진일보연구증실돌변위점여내약적관계。
Abstratc:Objective It is to investigate the characteristics of genetic mutations in 50S ribosomal protein gene of linezolid-induced Staphylococcus aureus in vitro . Methods Two S.aureus strain ATCC29213 and ATCC25923 named S2 and S3, two i-solated from the breast milk and blood ( named S5 and S7) were induced by linezolid with series dilution methods in vitro . Such four strains were sensitive to Linezolid and Methicillin .Four mother strains , and series of LZD -nonsusceptible ones were isolated and the MIC value was identified by E -Test.Then, the 50S ribosomal protein gene of these strains was ampli-fied and sequenced .Results 24 linezolid-nonsusceptible S .aureus induced in vitro was isolated and identified .Then, PCR and sequencing indicated the genetic mutations in 50S V ribosomal protein gene of these strains contained multiple point muta-tions in L3 ribosomal protein, while in L4, the common amino acid mutation was Ala56Asp except for S2 series strains.Con-clusion Linezolid-resistant S.aureus can be induced in vitro.Linezolid resistance in S.aureus was closely associated with the genetic mutations in 50S ribosomal protein gene , but the resistant mechanism needed to be further clarified .
