中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2015年
16期
7-7,9
,共2页
翼状胬肉%免疫组化%抗原修复
翼狀胬肉%免疫組化%抗原脩複
익상노육%면역조화%항원수복
Pterygium%Immunohistochemistry%Antigen repair
目的：分析3种抗原修复方法在翼状胬肉免疫组织化学染色过程中所得切片的优劣。方法：分别用3种不同抗原修复方法包括微波修复、酶修复和高压修复，对翼状胬肉组织蜡块的连续切片加抗TGF-ΒrⅡ抗体并染色。对所得切片的掉片结果进行分析。结果：3种方法间比较差异有统计学意义(P＜0.05)；微波修复与高压修复，酶修复与高压修复间比较差异有统计学意义(P＜0.05)。结论：对翼状胬肉组织进行组织抗原修复的最佳选择是酶修复法，掉片少，简单易行，且没有危险性。
目的：分析3種抗原脩複方法在翼狀胬肉免疫組織化學染色過程中所得切片的優劣。方法：分彆用3種不同抗原脩複方法包括微波脩複、酶脩複和高壓脩複，對翼狀胬肉組織蠟塊的連續切片加抗TGF-ΒrⅡ抗體併染色。對所得切片的掉片結果進行分析。結果：3種方法間比較差異有統計學意義(P＜0.05)；微波脩複與高壓脩複，酶脩複與高壓脩複間比較差異有統計學意義(P＜0.05)。結論：對翼狀胬肉組織進行組織抗原脩複的最佳選擇是酶脩複法，掉片少，簡單易行，且沒有危險性。
목적：분석3충항원수복방법재익상노육면역조직화학염색과정중소득절편적우렬。방법：분별용3충불동항원수복방법포괄미파수복、매수복화고압수복，대익상노육조직사괴적련속절편가항TGF-ΒrⅡ항체병염색。대소득절편적도편결과진행분석。결과：3충방법간비교차이유통계학의의(P＜0.05)；미파수복여고압수복，매수복여고압수복간비교차이유통계학의의(P＜0.05)。결론：대익상노육조직진행조직항원수복적최가선택시매수복법，도편소，간단역행，차몰유위험성。
Objective:To explore the advantages and disadvantages of received section of 3 antigen repair methods in the pterygium immunohistochemical dyeing process.Methods:The serial sections of pterygium organization wax block were given anti TGF-ΒrⅡ antibody and dye by using three different antigen repair methods,including microwave repair,enzymatic repair and high-pressure repair.Results:The differences were statistically significant between the three methods (P<0.05).The differences between microwave repair and high-pressure repair,enzymatic repair and high-pressure repair were statistically significant (P<0.05).Conclusion:The best choice of organization antigen repair for pterygium organization is enzymatic repair method.It has less chip shell.Iis simple and easy, and no danger.
