CAJ | 학술논문

目的：观察表没食子儿茶素没食子酸酯（ EGCG）处理后人脑胶质瘤U251细胞SHP1表达及其启动子区甲基化情况的变化，并探讨其对U251细胞增殖的影响及机制。方法以不同浓度的EGCG处理对数生长期的U251细胞，采用CCK-8法检测细胞增殖变化，RT-PCR及Western blotting法检测U251细胞SHP1基因及蛋白表达， MSP法检测EGCG处理后SHP1启动子区甲基化。结果经不同浓度EGCG处理后，U251细胞增殖被抑制，EGCG的抑制作用呈浓度依赖性；U251细胞中SHP1 mRNA的相对表达量及蛋白表达水平明显升高；SHP1启动子区的甲基化水平发生了翻转，由高甲基化转变为低甲基化。结论 EGCG可能通过引起人脑胶质瘤U251细胞SHP1启动子区去甲基化使SHP1表达增加，从而抑制细胞增殖。
목적：관찰표몰식자인다소몰식자산지（ EGCG）처리후인뇌효질류U251세포SHP1표체급기계동자구갑기화정황적변화，병탐토기대U251세포증식적영향급궤제。방법이불동농도적EGCG처리대수생장기적U251세포，채용CCK-8법검측세포증식변화，RT-PCR급Western blotting법검측U251세포SHP1기인급단백표체， MSP법검측EGCG처리후SHP1계동자구갑기화。결과경불동농도EGCG처리후，U251세포증식피억제，EGCG적억제작용정농도의뢰성；U251세포중SHP1 mRNA적상대표체량급단백표체수평명현승고；SHP1계동자구적갑기화수평발생료번전，유고갑기화전변위저갑기화。결론 EGCG가능통과인기인뇌효질류U251세포SHP1계동자구거갑기화사SHP1표체증가，종이억제세포증식。
Objective To observe the SHP1 expression changes and methylation in the promoter region of human glio-blastoma U251 cell line after the treatment of Epigallocatechin Gallate(EGCG), and to investigate the effect and mecha-nism of EGCG on the cell proliferation of U251 cell line.Methods U251 cells in the logarithmic phase were treated by different concentrations of EGCG.The cell proliferation was detected by CCK-8.The SHP1 gene and protein expression of U251 cells was evaluated by RT-PCR or Western blotting.MSP was used to testify methylation in the promoter region of SHP1 after EGCG treatment.Results After the treatment of different concentrations of EGCG, the proliferation of U251 cell was inhibited, and the inhibitory effect was in a concentration-dependent manner.The SHP1 mRNA and protein ex-pression was elevated in U251 cells, and the promoter methylation of SHP1 was reversed from hypermethylation to hypom-ethylation.Conclusions EGCG may increase SHP1 expression by causing the methylation in the promoter region of SHP1 of the human glioblastoma U251 cell line, and thus inhibit the proliferation of U251 cell.