现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
12期
1761-1763
,共3页
骨和骨组织%生物力学%生物相容性材料%碱性磷酸酶%二氧化钛%聚醚醚酮
骨和骨組織%生物力學%生物相容性材料%堿性燐痠酶%二氧化鈦%聚醚醚酮
골화골조직%생물역학%생물상용성재료%감성린산매%이양화태%취미미동
Bone and bones%Biomechanics%Biocompatible materials%Alkaline phosphatase%TiO2%Polyetheretherketone
目的：探讨TiO2颗粒喷砂处理聚醚醚酮（PEEK）的最佳粒度范围。方法用5种粒度范围的TiO2颗粒（A~E组）对PEEK进行喷砂处理，进行表面粗糙度检测，观察细胞黏附、细胞增殖、细胞形貌，并检测碱性磷酸酶（ALP）活性。结果 PEEK的表面粗糙度随着喷砂TiO2颗粒的粒度增大而增加。同时发现了多个峰值的存在，细胞黏附实验OD值的峰值出现在C组（180~212μm TiO2颗粒进行喷砂），数值为0.5662。经过14 d培养，成骨细胞ALP活性峰值出现在D组（>212~355μm TiO2颗粒进行喷砂）。结论 PEEK表面喷砂的最佳TiO2颗粒粒度范围为180~355μm，可为今后PEEK表面改性研究提供重要参考。
目的：探討TiO2顆粒噴砂處理聚醚醚酮（PEEK）的最佳粒度範圍。方法用5種粒度範圍的TiO2顆粒（A~E組）對PEEK進行噴砂處理，進行錶麵粗糙度檢測，觀察細胞黏附、細胞增殖、細胞形貌，併檢測堿性燐痠酶（ALP）活性。結果 PEEK的錶麵粗糙度隨著噴砂TiO2顆粒的粒度增大而增加。同時髮現瞭多箇峰值的存在，細胞黏附實驗OD值的峰值齣現在C組（180~212μm TiO2顆粒進行噴砂），數值為0.5662。經過14 d培養，成骨細胞ALP活性峰值齣現在D組（>212~355μm TiO2顆粒進行噴砂）。結論 PEEK錶麵噴砂的最佳TiO2顆粒粒度範圍為180~355μm，可為今後PEEK錶麵改性研究提供重要參攷。
목적：탐토TiO2과립분사처리취미미동（PEEK）적최가립도범위。방법용5충립도범위적TiO2과립（A~E조）대PEEK진행분사처리，진행표면조조도검측，관찰세포점부、세포증식、세포형모，병검측감성린산매（ALP）활성。결과 PEEK적표면조조도수착분사TiO2과립적립도증대이증가。동시발현료다개봉치적존재，세포점부실험OD치적봉치출현재C조（180~212μm TiO2과립진행분사），수치위0.5662。경과14 d배양，성골세포ALP활성봉치출현재D조（>212~355μm TiO2과립진행분사）。결론 PEEK표면분사적최가TiO2과립립도범위위180~355μm，가위금후PEEK표면개성연구제공중요삼고。
Objective To find out the best granularity range of TiO2 particles blasting process on polyether-ether-ketone (PEEK). Methods Five kinds of granule range of TiO2 particles(group A-E) were utilized for conducting the surface blasting pro-cess on PEEK. The surface roughness was tested,the cell attachment,cell proliferation and cell morphology were observed and alka-line phosphatase(ALP) activity was detected. Results The roughness of blasted PEEK surface was increased as the increase of TiO 2 particles granularity. At the same time several peak values were found. In the cell attachment test ,the peak OD value appeared in the group C(blasting by 180-212μm of TiO2 particles),which was 0.566 2. After 14 d culture,the peak value of osteoblast ALP activi-ty appeared in the group D(blasting by>212~355μm of TiO2 particles). Conclusion The best granularity range of TiO2 particles for PEEK surface blasting is 180-355μm,which can provide an important reference for the future study of PEEK surface modifi-cation in the future.
