CAJ | 학술논문

目的：观察HMGA2 siRNA对胆管癌RBE细胞生物学行为的影响。方法将常规培养的胆管癌RBE细胞分为观察组和对照组，每组6个复孔。观察组加入HMGA2 siRNA，对照组加入空白对照siRNA。采用CCK8法计算两组增殖速率。采用流式细胞术检测两组细胞凋亡率。采用细胞迁移实验检测两组细胞迁移能力。采用流式细胞数检测两组细胞周期分布。结果①细胞增殖速率：观察组、对照组细胞增殖速率分别为0．30±0．01、0．49±0．03观察组低于对照组，P＜0．05。②细胞凋亡率：观察组、对照组早期凋亡率分别为18．71％±0．61％、11．90％±1．01％，总凋亡率分别为25．73％±0．19％、18．66％±0．97％，观察组早期、总凋亡率均高于对照组，P均＜0．05。③细胞迁移能力：观察组、对照组穿膜细胞数分别为15．0±0．6、40．7±1．5，观察组低于对照组（ P＜0．05）。④细胞周期分布：观察组、对照组S期细胞比例分别为35．34％±1．95％、23．82％±0．95％，观察组高于对照组（P＜0．05）。结论 HMGA2 siRNA可以抑制胆管癌RBE细胞增殖和迁移、促进细胞凋亡、阻滞细胞周期阻于S期。
목적：관찰HMGA2 siRNA대담관암RBE세포생물학행위적영향。방법장상규배양적담관암RBE세포분위관찰조화대조조，매조6개복공。관찰조가입HMGA2 siRNA，대조조가입공백대조siRNA。채용CCK8법계산량조증식속솔。채용류식세포술검측량조세포조망솔。채용세포천이실험검측량조세포천이능력。채용류식세포수검측량조세포주기분포。결과①세포증식속솔：관찰조、대조조세포증식속솔분별위0．30±0．01、0．49±0．03관찰조저우대조조，P＜0．05。②세포조망솔：관찰조、대조조조기조망솔분별위18．71％±0．61％、11．90％±1．01％，총조망솔분별위25．73％±0．19％、18．66％±0．97％，관찰조조기、총조망솔균고우대조조，P균＜0．05。③세포천이능력：관찰조、대조조천막세포수분별위15．0±0．6、40．7±1．5，관찰조저우대조조（ P＜0．05）。④세포주기분포：관찰조、대조조S기세포비례분별위35．34％±1．95％、23．82％±0．95％，관찰조고우대조조（P＜0．05）。결론 HMGA2 siRNA가이억제담관암RBE세포증식화천이、촉진세포조망、조체세포주기조우S기。
Objective To observe the effect of HMGA2 on biological behavior of the cholangiocarcinoma cell of RBE. Methods The cholangiocarcinoma cell of RBE cultured by routine method were divided into observation group and control group, each group had six holes.HMGA2 siRNA and NC siRNA were added to observation group and control group.The proliferation rate of the two groups were determined by CCK8 assay.Cell cycle distribution and cell apoptosis rate were de-tectedbyflowcytometry.Migrateabilityofthetwogroupsweredetectedbycellmigrationexperiment.Results ①Cell proliferation rate:The cell proliferation rate of observation group and control group were 0.30 ±0.01, 0.49 ±0.03, obser-vation group was lower than the control group, P<0.05.② Apoptosis rate: The early apoptosis rate of observation group and control group were 18.71%±0.61%, 11.90%±1.01%, total apoptosis rate were 25.73%±0.19%, 18.66%± 0.97%, apoptosis rate of observation group was higher than that of control group, P<0.05.③Cell migration ability:The number of the cells across the membrane in observation group and control group were 15.0 ±0.6, 15.0 ±1.5, observation group was lower than the control group, P<0.05.④Cell cycle distribution:The cells in S phase of the observation group and control group were 35.34%±1.95%, 23.82%±0.95%, observation group was higher than the control group, P<0.05.Conclusions HMGA2 siRNA can inhibit the cell proliferation and migration, it can also induce cell apoptosis and block cell cycle in S phase in the cholangiocarcinoma cell of RBE.