CAJ | 학술논문

以转几丁质酶和葡聚糖酶双价基因棉花为研究对象，非转基因受体棉花为对照，通过比较可培养细菌数量和基于16S rRNA克隆文库细菌种群分析，评价外源双价基因的导入在苗期、蕾期、花铃期和吐絮期对棉花根际细菌群落多样性的影响。结果表明，可培养细菌的数量不受外源双价基因的影响，随着棉花生育期的交替而变化，以代谢旺盛的花铃期最多。构建的转基因和非转基因不同生育期根际土壤细菌16S rRNA文库容量为2400个克隆，涵盖了细菌的283个属。其中，Acidobacterium是最大优势类群，共包括624个克隆，其次为未知细菌种群和 Flavisolibacter。比较转基因和非转基因棉花根际土壤细菌的种群结构，结果显示，同一生育期内前者种群的多样性显著低于后者，二者的共有类群随着生长发育的进行而增多。研究结果说明几丁质酶基因和葡聚糖酶基因对棉花根际细菌种群多样性有着不同程度的削减作用，但是随着种植时间的延长，该差异呈现逐渐缩小的趋势。
이전궤정질매화포취당매쌍개기인면화위연구대상，비전기인수체면화위대조，통과비교가배양세균수량화기우16S rRNA극륭문고세균충군분석，평개외원쌍개기인적도입재묘기、뢰기、화령기화토서기대면화근제세균군락다양성적영향。결과표명，가배양세균적수량불수외원쌍개기인적영향，수착면화생육기적교체이변화，이대사왕성적화령기최다。구건적전기인화비전기인불동생육기근제토양세균16S rRNA문고용량위2400개극륭，함개료세균적283개속。기중，Acidobacterium시최대우세류군，공포괄624개극륭，기차위미지세균충군화 Flavisolibacter。비교전기인화비전기인면화근제토양세균적충군결구，결과현시，동일생육기내전자충군적다양성현저저우후자，이자적공유류군수착생장발육적진행이증다。연구결과설명궤정질매기인화포취당매기인대면화근제세균충군다양성유착불동정도적삭감작용，단시수착충식시간적연장，해차이정현축점축소적추세。
The transgenic cotton expressing chitinase and glucanase genes was studied using nontransgenic cotton as a control. Specifically, the effects of exogenous genes on bacterial community diversity in rhizospheres of cotton at stages of seedling, budding, boll forming and boll opening were evaluated through comparing the number of cultiva-ble bacteria and analyzing 16S rRNA gene clone libraries. The results showed that the number of cultivable bacteria was not affected by exogenous genes but the cotton growth period, and the number peaked at the stage of boll forming with vigorous metabolism. The 16S rRNA gene clone library prepared from soil bacteria in rhizospheres of transgenic and nontransgenic cotton at different stages contained 2400 clones which covered 283 genera. Among them,Acido-bacterium was the most dominant group which contained 642 clones, followed by unclassified bacterium and Flavisolibacter. Compared with nontransgenic cotton, the rhizosphere bacterial diversity of transgenic cotton exhib-ited lower level at the same growth stage, however, their common bacterial communities increased with growth and development. Our results suggest that chitinase and glucanase genes decrease the rhizosphere bacterial diversity at distinct degrees, however, the difference of bacterial diversity between transgenic and nontransgenic cotton reduces gradually with the extension of cultivation period.