CAJ | 학술논문

植物质体转化技术通过同源重组实现定点整合，与细胞核基因工程相比，使外源基因表达更为精确、安全和高效。该技术在基础研究中为叶绿体功能基因组研究提供了有效手段，同时在应用方面为外源基因表达提供了理想的平台，已成为植物遗传育种的一种新策略。本文总结了近年来质体基因工程在转化体系的建立和优化上的新思路，着重阐述了利用质体转化技术在遗传育种中提高作物抗性、改良品质等应用领域的最新研究进展。克服质体转化技术在作物遗传育种中面临的难题，必将为作物育种的发展带来新的绿色革命。
식물질체전화기술통과동원중조실현정점정합，여세포핵기인공정상비，사외원기인표체경위정학、안전화고효。해기술재기출연구중위협록체공능기인조연구제공료유효수단，동시재응용방면위외원기인표체제공료이상적평태，이성위식물유전육충적일충신책략。본문총결료근년래질체기인공정재전화체계적건립화우화상적신사로，착중천술료이용질체전화기술재유전육충중제고작물항성、개량품질등응용영역적최신연구진전。극복질체전화기술재작물유전육충중면림적난제，필장위작물육충적발전대래신적록색혁명。
The plastid genome engineering system allows site-specific modificationsvia two homologous recom-bination events. It is much safer, more precise and efficient compared with the nuclear transformation system. This technology can be applied to the basic research to expand plastid genome function analysis, and it also provides an excellent platform for not only high-level production of recombinant proteins but also plant breeding. In this review, we summarize the state of the art and progresses in this field. We focus on novel breeding strategies in transformation system improvement and new tools to enhance plastid transgene expression levels. In addition, we highlight selected applications in resistance engineering and quality improvementvia metabolic engineering. We believe that by over-coming current technological limitations in the plastid transformation system can another green revolution for crop breeding beckon.