CAJ | 학술논문

为了深入了解绿色杜氏藻(Dunaliella viridis)基因信息及功能、耐盐相关通路(甘油脂代谢)及关键酶，本文首次通过Illumina HiSeqTM 2000高通量测序技术对绿色杜氏藻转录组进行测序，利用Trinity软件将数据组装形成转录本，对所有转录本进行COG(Clusters of Orthologous Groups)、GO(Gene Ontology)和KEGG(Kyoto En-cyclopedia of Genes and Genomes)分类和功能注释、Pathway注释以及蛋白编码区(Opening reading fragment，ORF)的预测，并对甘油脂代谢通路关键酶基因进行了分析。转录组测序共获得81593个转录本，其中ORF共有77117条，约占所有转录本的94.50%。COG分类结果表明，16569条转录本被分为24个类别。GO分类结果表明，76436条转录本被注释。在所有注释分类中，生物学过程转录本数量最多，为30678条，占总转录本数的40.14%。KEGG分析结果表明，317个标准途径中包含26428条转录本，含转录本最多的类别是代谢，为9949条(37.65%)。与代谢有关的途径为131条，占所有注释途径的41.32%。在甘油脂代谢通路中仅发现1条关键酶转录本(二羟丙酮激酶)，该酶可能与绿色杜氏藻耐盐胁迫中甘油的合成有较大关系。本研究进一步完善了绿色杜氏藻的基因信息，为绿色杜氏藻代谢途径研究奠定了坚实的基础。
위료심입료해록색두씨조(Dunaliella viridis)기인신식급공능、내염상관통로(감유지대사)급관건매，본문수차통과Illumina HiSeqTM 2000고통량측서기술대록색두씨조전록조진행측서，이용Trinity연건장수거조장형성전록본，대소유전록본진행COG(Clusters of Orthologous Groups)、GO(Gene Ontology)화KEGG(Kyoto En-cyclopedia of Genes and Genomes)분류화공능주석、Pathway주석이급단백편마구(Opening reading fragment，ORF)적예측，병대감유지대사통로관건매기인진행료분석。전록조측서공획득81593개전록본，기중ORF공유77117조，약점소유전록본적94.50%。COG분류결과표명，16569조전록본피분위24개유별。GO분류결과표명，76436조전록본피주석。재소유주석분류중，생물학과정전록본수량최다，위30678조，점총전록본수적40.14%。KEGG분석결과표명，317개표준도경중포함26428조전록본，함전록본최다적유별시대사，위9949조(37.65%)。여대사유관적도경위131조，점소유주석도경적41.32%。재감유지대사통로중부발현1조관건매전록본(이간병동격매)，해매가능여록색두씨조내염협박중감유적합성유교대관계。본연구진일보완선료록색두씨조적기인신식，위록색두씨조대사도경연구전정료견실적기출。
In order to understand the gene information, function, haloduric pathway (glycerolipid metabolism) and related key genes forDunaliella viridis, we used Illumina HiSeqTM 2000 high-throughput sequencing technology to sequence its transcriptome. Trinity soft was used to assemble the data to form transcripts. Based on the Clusters of Orthologous Groups (COG), Gene Ontology (GO ) and Kyoto Encyclopedia of Genes and Genomes (KEGG ) data-bases, we carried out functional annotation and classification, pathway annotation, and the opening reading fragment (ORF) sequence prediction of transcripts. The key genes in the glycerolipid metabolism were analyzed. The results suggested that 81 593 transcripts were found, and 77 117 ORF sequences were predicted, accounting for 94.50% of all transcripts. COG classification results showed that 16 569 transcripts were assigned to 24 categories. GO classifica-tion annotated 76 436 transcripts. The number of transcripts for biologcial processes was 30 678, accounting for 40.14% of all transcripts. KEGG pathway analysis showed that 26 428 transcripts were annotated to 317 pathways, and 131 pathways were related to metabolism, accounting for 41.32% of all annotated pathways. Only one transcript was an-notated as coding the key enzyme dihydroxyacetone kinase involved in the glycerolipid pathway. This enzyme could be related to glycerol biosynthesis under salt stress. This study further improved the gene information and laid the foun-dation of metabolic pathway research forDunaliella viridis.