遗传
遺傳
유전
HEREDITAS(BEIJING)
2015年
8期
811-822
,共12页
张涛%王文浩%张跟喜%王金玉%薛倩%顾玉萍
張濤%王文浩%張跟喜%王金玉%薛倩%顧玉萍
장도%왕문호%장근희%왕금옥%설천%고옥평
京海黄鸡%全基因组关联分析%体重性状%简化基因组测序
京海黃鷄%全基因組關聯分析%體重性狀%簡化基因組測序
경해황계%전기인조관련분석%체중성상%간화기인조측서
Jinghai yellow chicken%GWAS%body weight traits%SLAF-seq
体重性状是肉鸡重要的经济性状。为了寻找可用于京海黄鸡体重性状遗传改良的分子标记及候选基因,本文以400只京海黄鸡核心群母鸡为基础,测定了0~14周龄体重,利用简化基因组测序技术(Specific-locus amplified fragment sequencing, SLAF-seq)对京海黄鸡体重性状进行全基因组关联研究(Genome-wide association stndy, GWAS),筛选与京海黄鸡体重性状相关的SNPs位点。结果共检测到100个与京海黄鸡体重相关的SNPs位点,其中15个位点效应达到全基因组显著水平(P<1.87E-06),85个位点效应达到全基因组潜在显著水平(P<3.73E-05)。通过筛选每个显著SNP周围1 Mb区域内的基因,共找到9个可能的候选基因,其中FAM124A(Family with sequence similarity 124A)、QDPR(Quinoid dihydropteridine reductase)、WDR1(WD repeat domain 1)和SLC2A9(Solute carrier family 2(facilitated glucose transporter), member 9)4个基因可能是影响体重性状的重要候选基因。同时还发现,4号染色体75.6~80.7 Mb区域集中了大部分与京海黄鸡中后期体重性状显著相关的SNPs位点,该区域可能是影响京海黄鸡中后期生长体重的重要候选区域。
體重性狀是肉鷄重要的經濟性狀。為瞭尋找可用于京海黃鷄體重性狀遺傳改良的分子標記及候選基因,本文以400隻京海黃鷄覈心群母鷄為基礎,測定瞭0~14週齡體重,利用簡化基因組測序技術(Specific-locus amplified fragment sequencing, SLAF-seq)對京海黃鷄體重性狀進行全基因組關聯研究(Genome-wide association stndy, GWAS),篩選與京海黃鷄體重性狀相關的SNPs位點。結果共檢測到100箇與京海黃鷄體重相關的SNPs位點,其中15箇位點效應達到全基因組顯著水平(P<1.87E-06),85箇位點效應達到全基因組潛在顯著水平(P<3.73E-05)。通過篩選每箇顯著SNP週圍1 Mb區域內的基因,共找到9箇可能的候選基因,其中FAM124A(Family with sequence similarity 124A)、QDPR(Quinoid dihydropteridine reductase)、WDR1(WD repeat domain 1)和SLC2A9(Solute carrier family 2(facilitated glucose transporter), member 9)4箇基因可能是影響體重性狀的重要候選基因。同時還髮現,4號染色體75.6~80.7 Mb區域集中瞭大部分與京海黃鷄中後期體重性狀顯著相關的SNPs位點,該區域可能是影響京海黃鷄中後期生長體重的重要候選區域。
체중성상시육계중요적경제성상。위료심조가용우경해황계체중성상유전개량적분자표기급후선기인,본문이400지경해황계핵심군모계위기출,측정료0~14주령체중,이용간화기인조측서기술(Specific-locus amplified fragment sequencing, SLAF-seq)대경해황계체중성상진행전기인조관련연구(Genome-wide association stndy, GWAS),사선여경해황계체중성상상관적SNPs위점。결과공검측도100개여경해황계체중상관적SNPs위점,기중15개위점효응체도전기인조현저수평(P<1.87E-06),85개위점효응체도전기인조잠재현저수평(P<3.73E-05)。통과사선매개현저SNP주위1 Mb구역내적기인,공조도9개가능적후선기인,기중FAM124A(Family with sequence similarity 124A)、QDPR(Quinoid dihydropteridine reductase)、WDR1(WD repeat domain 1)화SLC2A9(Solute carrier family 2(facilitated glucose transporter), member 9)4개기인가능시영향체중성상적중요후선기인。동시환발현,4호염색체75.6~80.7 Mb구역집중료대부분여경해황계중후기체중성상현저상관적SNPs위점,해구역가능시영향경해황계중후기생장체중적중요후선구역。
Body weight traits are important economic characters of broilers. This study was carried out to screen for molecular markers and candidate genes that can be used to improve the body weight traits. A herd of 400 female Jinghai yellow chickens were measured for body weights from 0 to 14 weeks of age. Genome-wide association study (GWAS) was carried out using specific-locus amplified fragment sequencing (SLAF-seq) technology to detect SNPs associated with body weight traits of Jinghai yellow chicken. Finally, 100 SNPs that associated with body weight traits were detected. The results showed that effects of 15 SNPs reached 5% Bonferroni genome-wide significance and 85 SNPs reached potential genome-wide significance. Genes in the candidate regions with 1 Mb windows (SNP position ±0.5 Mb) surrounding each significant SNP were screened. Finally, nine candidate genes were obtained, among which four genes ofFAM124A (Family with sequence similarity 124A),QDPR (Quinoid dihydropteridine reductase),WDR1 (WD repeat domain 1) and SLC2A9 (Solute carrier family 2 (facilitated glucose transporter), member 9) might be important candidate genes influencing body weight traits of Jinghai yellow chicken. Furthermore, it was also found that most SNPs associated with mid and late growth and body weights were intensively located in the region of 75.6?80.7 Mb on chromosome 4. Our study thus provides a basis for genetic understanding of the Jing-hai yellow chicken body weight traits.