CAJ | 학술논문

目的：研究番茄红素（ Lycopene ， LP ）对实验性糖尿病大鼠肾脏的保护作用。方法首先通过高脂高糖饮食饲养8周后腹腔注射链脲佐菌素（ STZ）方法制备糖尿病大鼠模型，将造模成功的80只大鼠随机分为模型组、番茄红素5 mg／kg组、番茄红素10 mg／kg组、番茄红素20 mg／kg组和盐酸二甲双胍组，每组16只。另取16只健康大鼠作为正常对照组。正常对照组和模型组连续灌胃生理盐水，番茄红素各组和盐酸二甲双胍组分别连续灌胃相应剂量番茄红素和盐酸二甲双胍（200 mg／kg），每天1次，连续28 d。分别于给药前和给药后第7，14，28天测定各组大鼠胰岛素和空腹血糖水平。给药28 d后，观察各组大鼠一般状况，测定各组大鼠体质量、肾脏指数、24 h尿量、24 h尿蛋白量（ UPro ）及血尿素氮（ BUN ）、肌酐（SCr）、尿酸（UA）水平；测定各组大鼠肾脏组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH－Px）活性和丙二醛（ MDA）含量；通过PAS染色观察肾脏组织病理形态学变化；通过TUNEL染色观察肾小球细胞凋亡状况，并计算凋亡指数（ AI）。结果番茄红素各组大鼠一般状况得到明显改善，其中以20 mg／kg组效果最为显著。番茄红素10 mg／kg组灌胃第28天胰岛素水平明显高于模型组，番茄红素20 mg／kg组灌胃第14，21，28天胰岛素水平均明显高于模型组。番茄红素20 mg／kg组灌胃第21，28天空腹血糖水平均显著低于模型组。番茄红素10 mg／kg组和番茄红素20 mg／kg组大鼠体质量显著高于模型组，肾脏指数、尿量、UPro及血清BUN、SCr、UA水平均显著低于模型组；肾脏组织中SOD活性显著高于模型组，MDA含量显著低于模型组，其中番茄红素20 mg／kg组GSH－Px活性显著高于模型组。番茄红素各组大鼠肾脏组织病理形态学改变和肾脏组织细胞凋亡状况均明显改善，其中以20 mg／kg组效果最为显著；并且番茄红素10 mg／kg组和番茄红素20 mg／kg组大鼠肾小球细胞凋亡指数显著低于模型组。结论番茄红素对实验性糖尿病大鼠肾脏组织具有保护作用。目的：研究番茄紅素（ Lycopene ， LP ）對實驗性糖尿病大鼠腎髒的保護作用。方法首先通過高脂高糖飲食飼養8週後腹腔註射鏈脲佐菌素（ STZ）方法製備糖尿病大鼠模型，將造模成功的80隻大鼠隨機分為模型組、番茄紅素5 mg／kg組、番茄紅素10 mg／kg組、番茄紅素20 mg／kg組和鹽痠二甲雙胍組，每組16隻。另取16隻健康大鼠作為正常對照組。正常對照組和模型組連續灌胃生理鹽水，番茄紅素各組和鹽痠二甲雙胍組分彆連續灌胃相應劑量番茄紅素和鹽痠二甲雙胍（200 mg／kg），每天1次，連續28 d。分彆于給藥前和給藥後第7，14，28天測定各組大鼠胰島素和空腹血糖水平。給藥28 d後，觀察各組大鼠一般狀況，測定各組大鼠體質量、腎髒指數、24 h尿量、24 h尿蛋白量（ UPro ）及血尿素氮（ BUN ）、肌酐（SCr）、尿痠（UA）水平；測定各組大鼠腎髒組織中超氧化物歧化酶（SOD）、穀胱甘肽過氧化物酶（GSH－Px）活性和丙二醛（ MDA）含量；通過PAS染色觀察腎髒組織病理形態學變化；通過TUNEL染色觀察腎小毬細胞凋亡狀況，併計算凋亡指數（ AI）。結果番茄紅素各組大鼠一般狀況得到明顯改善，其中以20 mg／kg組效果最為顯著。番茄紅素10 mg／kg組灌胃第28天胰島素水平明顯高于模型組，番茄紅素20 mg／kg組灌胃第14，21，28天胰島素水平均明顯高于模型組。番茄紅素20 mg／kg組灌胃第21，28天空腹血糖水平均顯著低于模型組。番茄紅素10 mg／kg組和番茄紅素20 mg／kg組大鼠體質量顯著高于模型組，腎髒指數、尿量、UPro及血清BUN、SCr、UA水平均顯著低于模型組；腎髒組織中SOD活性顯著高于模型組，MDA含量顯著低于模型組，其中番茄紅素20 mg／kg組GSH－Px活性顯著高于模型組。番茄紅素各組大鼠腎髒組織病理形態學改變和腎髒組織細胞凋亡狀況均明顯改善，其中以20 mg／kg組效果最為顯著；併且番茄紅素10 mg／kg組和番茄紅素20 mg／kg組大鼠腎小毬細胞凋亡指數顯著低于模型組。結論番茄紅素對實驗性糖尿病大鼠腎髒組織具有保護作用。
목적：연구번가홍소（ Lycopene ， LP ）대실험성당뇨병대서신장적보호작용。방법수선통과고지고당음식사양8주후복강주사련뇨좌균소（ STZ）방법제비당뇨병대서모형，장조모성공적80지대서수궤분위모형조、번가홍소5 mg／kg조、번가홍소10 mg／kg조、번가홍소20 mg／kg조화염산이갑쌍고조，매조16지。령취16지건강대서작위정상대조조。정상대조조화모형조련속관위생리염수，번가홍소각조화염산이갑쌍고조분별련속관위상응제량번가홍소화염산이갑쌍고（200 mg／kg），매천1차，련속28 d。분별우급약전화급약후제7，14，28천측정각조대서이도소화공복혈당수평。급약28 d후，관찰각조대서일반상황，측정각조대서체질량、신장지수、24 h뇨량、24 h뇨단백량（ UPro ）급혈뇨소담（ BUN ）、기항（SCr）、뇨산（UA）수평；측정각조대서신장조직중초양화물기화매（SOD）、곡광감태과양화물매（GSH－Px）활성화병이철（ MDA）함량；통과PAS염색관찰신장조직병리형태학변화；통과TUNEL염색관찰신소구세포조망상황，병계산조망지수（ AI）。결과번가홍소각조대서일반상황득도명현개선，기중이20 mg／kg조효과최위현저。번가홍소10 mg／kg조관위제28천이도소수평명현고우모형조，번가홍소20 mg／kg조관위제14，21，28천이도소수평균명현고우모형조。번가홍소20 mg／kg조관위제21，28천공복혈당수평균현저저우모형조。번가홍소10 mg／kg조화번가홍소20 mg／kg조대서체질량현저고우모형조，신장지수、뇨량、UPro급혈청BUN、SCr、UA수평균현저저우모형조；신장조직중SOD활성현저고우모형조，MDA함량현저저우모형조，기중번가홍소20 mg／kg조GSH－Px활성현저고우모형조。번가홍소각조대서신장조직병리형태학개변화신장조직세포조망상황균명현개선，기중이20 mg／kg조효과최위현저；병차번가홍소10 mg／kg조화번가홍소20 mg／kg조대서신소구세포조망지수현저저우모형조。결론번가홍소대실험성당뇨병대서신장조직구유보호작용。
Objective It is to investigate the protective effects of Lycopene ( LP) on the kidneys in experimental diabetic rats.Methods 80 diabetic rat models made by intraperitoneal injecting STZ after feeding with high fat and sugar diet for 8 weeks were randomly devided into 5 groups:model group, LP 5, 10, 20 mg/kg groups and Metformin hydrochloride group , each group had 16 rats.Another 16 same-aged rats were selected as normal control group .Normal control group and model group were given normal saline by intragastric administration , every LP group and Metformin hydrochloride group were treated with respective dosage of LP or Metformin hydrochloride (200mg/kg) once a day and for 28 days.Before and once week after the drug was given , the levels of insulin and blood sugar were determined .Four weeks later , the general status of the rats were observed;the body weight, renal index(RI), 24h urine volume, UPro and the content of BUN, SCr, UA in serum were deter-mined;the activity of SOD , GSH-Px and the content of MDA in renal tissue were determined;the renal tissue histo-patho-logical changes was observed by HE staining;the apoptosis of renal cells was observed by TUNEL staining , and the apoptosis index(AI) was alculated.Results Compared with diabetic model group , the general status of the rats in LP treated groups were improved, especially in 20mg/kg group the improvements were more significant; the level of insulin in LP 10 mg/kg group was significantly higher than that in model group on the 28th day, while the levels in 20 mg/kg group on the 14th, 21th, 28th day were all higher than that in model group .The blood sugar level of LP 20 mg/kg group was significantly lower than that in model group on the 21th and 28th days;the body weight and SOD activity in LP 10, 20 mg/kg groups were significantly higher, while RI, urine volume, UPro and the levels of serum BUN, SCr, UA, MDA were lower than that in model group . The activity of GSH-Px of LP 20 mg/kg group was significantly higher than that in model group .The histopathological chan-ges and the apoptosis of the renal tissue in LP treated groups were significantly improved , especially in LP 20 mg/kg group the improvements were more significant , and AI of LP 10, 20 mg/kg groups were significantly lower than than in model group . Conclusion LP had dose-dependent protective effects on kidneys in experiment diabetic rats .