山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
30期
17-19
,共3页
涂洋%陈光亮%曹珊%陈晓翔
塗洋%陳光亮%曹珊%陳曉翔
도양%진광량%조산%진효상
系统性红斑狼疮%α2,8-唾液酸转移酶%微小核糖核酸%聚合酶链反应%基因芯片
繫統性紅斑狼瘡%α2,8-唾液痠轉移酶%微小覈糖覈痠%聚閤酶鏈反應%基因芯片
계통성홍반랑창%α2,8-타액산전이매%미소핵당핵산%취합매련반응%기인심편
systemic lupus erythematosus%α2,8-sialyltransferases%miRNA%polymerase chain reaction%microarray
目的：观察系统性红斑狼疮（SLE）患者外周血白细胞唾液酸转移酶（ST8SIA）的表达变化，并探讨其机制。方法选择SLE患者（观察组）和健康志愿者（对照组）各24例，采用RT-PCR法检测外周血白细胞ST8SIA1～6 mRNA；对有统计学意义的ST8SIA mRNA，采用基因芯片技术分析血浆中靶向其3′非翻译区（3′UTR）的miR-NA。结果两组外周血白细胞均无ST8SIA2、ST8SIA3、ST8SIA5 mRNA表达；观察组ST8SIA1、4、6 mRNA相对表达量分别为1．524±0．229、13．780±1．669、2．238±0．387，对照组分别为1．129±0．106、1．087±0．089、0．550±0．054；两组ST8SIA4、6 mRNA比较，P均＜0．05。靶向调节ST8SIA4 mRNA的miRNA表达下降12条、升高1条，靶向调节ST8SIA6 mRNA的miRNA表达下降、升高各4条。结论 SLE患者外周血白细胞中ST8SIA4、ST8SIA6 mR-NA表达升高，与对其进行靶向调控的miRNA表达异常有关。
目的：觀察繫統性紅斑狼瘡（SLE）患者外週血白細胞唾液痠轉移酶（ST8SIA）的錶達變化，併探討其機製。方法選擇SLE患者（觀察組）和健康誌願者（對照組）各24例，採用RT-PCR法檢測外週血白細胞ST8SIA1～6 mRNA；對有統計學意義的ST8SIA mRNA，採用基因芯片技術分析血漿中靶嚮其3′非翻譯區（3′UTR）的miR-NA。結果兩組外週血白細胞均無ST8SIA2、ST8SIA3、ST8SIA5 mRNA錶達；觀察組ST8SIA1、4、6 mRNA相對錶達量分彆為1．524±0．229、13．780±1．669、2．238±0．387，對照組分彆為1．129±0．106、1．087±0．089、0．550±0．054；兩組ST8SIA4、6 mRNA比較，P均＜0．05。靶嚮調節ST8SIA4 mRNA的miRNA錶達下降12條、升高1條，靶嚮調節ST8SIA6 mRNA的miRNA錶達下降、升高各4條。結論 SLE患者外週血白細胞中ST8SIA4、ST8SIA6 mR-NA錶達升高，與對其進行靶嚮調控的miRNA錶達異常有關。
목적：관찰계통성홍반랑창（SLE）환자외주혈백세포타액산전이매（ST8SIA）적표체변화，병탐토기궤제。방법선택SLE환자（관찰조）화건강지원자（대조조）각24례，채용RT-PCR법검측외주혈백세포ST8SIA1～6 mRNA；대유통계학의의적ST8SIA mRNA，채용기인심편기술분석혈장중파향기3′비번역구（3′UTR）적miR-NA。결과량조외주혈백세포균무ST8SIA2、ST8SIA3、ST8SIA5 mRNA표체；관찰조ST8SIA1、4、6 mRNA상대표체량분별위1．524±0．229、13．780±1．669、2．238±0．387，대조조분별위1．129±0．106、1．087±0．089、0．550±0．054；량조ST8SIA4、6 mRNA비교，P균＜0．05。파향조절ST8SIA4 mRNA적miRNA표체하강12조、승고1조，파향조절ST8SIA6 mRNA적miRNA표체하강、승고각4조。결론 SLE환자외주혈백세포중ST8SIA4、ST8SIA6 mR-NA표체승고，여대기진행파향조공적miRNA표체이상유관。
Objective To observe the expression changes of sialyltransferase ( ST8SIA) in the peripheral blood leucocytes of patients with systemic lupus erythematosus ( SLE) and to investigate the possible mechanism.Methods ST8SIA1-6 was detected by RT-PCR in the peripheral blood leucocytes in 24 SLE patients and 24 normal donors. We analyzed the miRNAs that targeting 3`untranslated region ( UTR) by microarray chip.Results The expression of ST8 SIA2 , ST8 SIA3 and ST8 SIA5 was not detected in the peripheral blood leucocytes of the two groups .The relative expression levels of ST8SIA1 mRNA, ST8SIA4 mRNA and ST8SIA6 mRNA in the observation group were 1.524 ± 0.229, 13.78 ±1.669 and 2.238 ±0.387, and they were 1.129 ±0.106, 1.087 ±0.089 and 0.550 ±0.054 in the control group.The difference in ST8 SIA4 mRNA and ST8 SIA6 mRNA of the two groups was statistically different ( all P<0.05).There were 12 down-regulated plasma miRNAs that regulated the 3`UTR of ST8SIA4 mRNA and 1 up-regulated plasma miRNA.There were 4 plasma miRNAs that made the 3`UTR of ST8SIA6 mRNA down-regulated and 4 plasma miRNAs up-regulated.Conclusion The expression levels of ST8SIA4 mRNA and ST8SIA6 mRNA in patients with SLE are increased, which may be related with the abnormal expression of plasma miRNAs that can regu-late the 3`UTR of ST8SIA4 mRNA and ST8SIA6 mRNA.
