CAJ | 학술논문

目的：观察自限性和慢性乙型肝炎病毒（HBV）感染患者干扰素ω1（IFN-ω1）、干扰素γ受体（IFNGR）基因的单核苷酸多态性（ SNP）。方法应用聚合酶链反应-限制性片段长度多态性分析方法检测206例自限性HBV感染者（对照组）和210例慢性HBV感染者（病例组） IFN-ω1基因上4个SNP位点、IFNGR基因上4个位点，比较两组基因型和等位基因频率。结果病例组、对照组IFNGR基因的rs9376267位点CT基因型频率分别为48．4％、60．3％，两组比较，P＝0．012。两组IFN-ω1、IFNGR基因其他SNP位点基因型和等位基因频率比较差异无统计学意义。结论自限性HBV感染患者IFNGR基因上的rs9376267位点CT基因型频率较慢性HBV感染患者高。
목적：관찰자한성화만성을형간염병독（HBV）감염환자간우소ω1（IFN-ω1）、간우소γ수체（IFNGR）기인적단핵감산다태성（ SNP）。방법응용취합매련반응-한제성편단장도다태성분석방법검측206례자한성HBV감염자（대조조）화210례만성HBV감염자（병례조） IFN-ω1기인상4개SNP위점、IFNGR기인상4개위점，비교량조기인형화등위기인빈솔。결과병례조、대조조IFNGR기인적rs9376267위점CT기인형빈솔분별위48．4％、60．3％，량조비교，P＝0．012。량조IFN-ω1、IFNGR기인기타SNP위점기인형화등위기인빈솔비교차이무통계학의의。결론자한성HBV감염환자IFNGR기인상적rs9376267위점CT기인형빈솔교만성HBV감염환자고。
Objective To observe the gene single nucleotide polymorphisms (SNPs) of interferon-ω1 (IFN-ω1) and interferon-γreceptor ( IFNGR) in self-limiting hepatitis B virus ( HBV) infection and chronic HBV infection patients. Methods PCR-RFLP was used to detect four SNP sites of IFN-ω1 and four sites of IFNGR in 206 cases of patients with self-limiting HBV infection ( control group) and 210 cases of patients with chronic HBV infection ( case group) .The geno-type and allele frequency of the two groups were compared.Results The rs9376267 gene loci CT genotype frequencies of IFNGR in the case group and control group were respectively 48.4%and 60.3%(P=0.012).No statistically significant difference was found between the two groups in the genotype and allele frequency of other SNP sites of IFN-ω1 and IFNGR genes.Conclusion The CT genotype frequency on IFNGR rs9376267 site in patients with self-limiting HBV infection was higher than that of the patients with chronic HBV infection.