CAJ | 학술논문

目的：探讨DLC-1和cyclinD1在宫颈鳞状细胞癌中的表达。方法整群选择2005年1月-2013年12月该院收治的62例宫颈鳞状细胞癌标本、38例宫颈上皮内瘤变标本、26例正常宫颈组织标本，以免疫组化SP法检测3组标本中DLC-1和cyclinD1的表达。结果DLC-1在宫颈鳞状细胞癌、宫颈上皮内瘤变、正常宫颈组织中的阳性表达率分别为27.4%、76.3%、92.3%，差异有统计学意义(P<0.05)；高、中、低病理分级DLC-1阳性表达率分别为42.9%、27.9%、16.7%，差异有统计学意义(P<0.05)；Ⅰ~Ⅱa期DLC-1阳性表达率为41.4%，明显高于Ⅱb~Ⅳ期的15.2%，差异有统计学意义(P<0.05)；无淋巴结转移DLC-1阳性表达率为34.9%，明显高于有淋巴结转移的10.5%，差异有统计学意义(P<0.05)。 cyclinD1在宫颈鳞状细胞癌、宫颈上皮内瘤变、正常宫颈组织中的阳性表达率分别为66.1%、5.3%、0%，差异有统计学意义(P<0.05)；高、中、低病理分级cy-clinD1阳性表达率分别为42.9%、67.4%、75.0%，差异有统计学意义(P<0.05)；Ⅰ~Ⅱa期cyclinD1阳性表达率为48.3%，明显低于Ⅱb~Ⅳ期的81.8%，差异有统计学意义(P<0.05)；无淋巴结转移cyclinD1阳性表达率为58.1%，明显低于有淋巴结转移的84.2%，差异有统计学意义(P<0.05)。 DLC-1与cyclinD1在宫颈鳞状细胞癌中的表达呈负相关性(r=-0.519，P<0.05)。结论DLC-1在宫颈鳞状细胞癌的阳性表达较低，而cyclinD1在宫颈鳞状细胞癌的阳性表达较高，两者均与宫颈鳞状细胞癌发展、侵袭、转移等密切相关。
목적：탐토DLC-1화cyclinD1재궁경린상세포암중적표체。방법정군선택2005년1월-2013년12월해원수치적62례궁경린상세포암표본、38례궁경상피내류변표본、26례정상궁경조직표본，이면역조화SP법검측3조표본중DLC-1화cyclinD1적표체。결과DLC-1재궁경린상세포암、궁경상피내류변、정상궁경조직중적양성표체솔분별위27.4%、76.3%、92.3%，차이유통계학의의(P<0.05)；고、중、저병리분급DLC-1양성표체솔분별위42.9%、27.9%、16.7%，차이유통계학의의(P<0.05)；Ⅰ~Ⅱa기DLC-1양성표체솔위41.4%，명현고우Ⅱb~Ⅳ기적15.2%，차이유통계학의의(P<0.05)；무림파결전이DLC-1양성표체솔위34.9%，명현고우유림파결전이적10.5%，차이유통계학의의(P<0.05)。 cyclinD1재궁경린상세포암、궁경상피내류변、정상궁경조직중적양성표체솔분별위66.1%、5.3%、0%，차이유통계학의의(P<0.05)；고、중、저병리분급cy-clinD1양성표체솔분별위42.9%、67.4%、75.0%，차이유통계학의의(P<0.05)；Ⅰ~Ⅱa기cyclinD1양성표체솔위48.3%，명현저우Ⅱb~Ⅳ기적81.8%，차이유통계학의의(P<0.05)；무림파결전이cyclinD1양성표체솔위58.1%，명현저우유림파결전이적84.2%，차이유통계학의의(P<0.05)。 DLC-1여cyclinD1재궁경린상세포암중적표체정부상관성(r=-0.519，P<0.05)。결론DLC-1재궁경린상세포암적양성표체교저，이cyclinD1재궁경린상세포암적양성표체교고，량자균여궁경린상세포암발전、침습、전이등밀절상관。
Objective To investigate the expression of DLC-1 and cyclinD1 in cervical squamous cell carcinoma. Methods 62 cases of cervical squamous cell carcinoma samples, 38 cases of cervical intraepithelial neoplasia samples, 26 cases of normal cer-vical tissue samples from January 2005 to December 2013 were selected, and the expression of DLC-1 and cyclinD1 were detect-ed by immunohistochemistry SP method. Results The positive expression of DLC-1 in cervical squamous cell carcinoma, cervical intraepithelial neoplasia, and normal cervical tissue, were 27.4%, 76.3%, and 92.3% respectively, the difference was statistically significant (P<0.05);The positive expression of DLC-1 in high, middle, and low pathological grade were 42.9%、27.9%、16.7%re-spectively, the difference was statistically significant (P<0.05);The positive expression of DLC-1 inⅠ~IIa was 41.4%, which was significantly higher thanIIb~Ⅳ15.2%(P<0.05); The positive expression of DLC-1 in patients without lymph node metastasis was 34.9%, which was significantly higher than patients with lymph node metastasis 10.5%(P<0.05). The positive expression of cy-clinD1 in cervical squamous cell carcinoma, cervical intraepithelial neoplasia, and normal cervical tissue, were 66.1%, 5.3%, 0%respectively, the difference was statistically significant (P<0.05); The positive expression of cyclinD1 in high, middle, and low pathological grade were 42.9%, 67.4%, 75.0% respectively, the difference was statistically significant (P<0.05); The positive ex-pression of cyclinD1 inⅠ~IIa was 48.3%, which was significantly higher thanIIb~Ⅳ81.8% (P<0.05);The positive expression of cyclinD1 in patients without lymph node metastasis was 58.1%, which was significantly higher than patients with lymph node metastasis 84.2%(P<0.05).The expression of DLC-1 and cyclinD1 in cervical squamous cell carcinoma showed a negative correla-tion (r=-0.519, P<0.05). Conclusion The expression of DLC-1 in squamous cell carcinoma is lower, and expression of cyclinD1 in squamous cell carcinoma is higher, and both of them are closely related to the stage, grade, and lymph node metastasis of squa-mous cell carcinoma.