CAJ | 학술논문

采用反相超效液相色谱-质谱联用的方法分离分析食用油中的甘油三酯。使用总长40 cm的串联超效C18色谱柱(10 cm+15 cm+15 cm),以乙腈-异丙醇(50：50, V/V)为流动相,流速0.2 mL/min,柱温25℃,在大约74 MPa的高柱压下分离,APCI离子化-质谱检测。食用油以异丙醇溶解后直接进样。实现了对食用油中甘油三酯组分的更精细区分,获得了玉米油、大豆油、花生油、葵花籽油、稻米油、橄榄油和芝麻油等市售食用油中甘油三酯组成的谱图。结果显示,甘油三酯在相同食用油中的组成相似,而在不同食用油中的组成存在差异。采用本方法,通过差异的色谱峰可直接识别出大豆油中掺入的5％猪油。本方法为识别掺假食用油提供了有价值的途径。
채용반상초효액상색보-질보련용적방법분리분석식용유중적감유삼지。사용총장40 cm적천련초효C18색보주(10 cm+15 cm+15 cm),이을정-이병순(50：50, V/V)위류동상,류속0.2 mL/min,주온25℃,재대약74 MPa적고주압하분리,APCI리자화-질보검측。식용유이이병순용해후직접진양。실현료대식용유중감유삼지조분적경정세구분,획득료옥미유、대두유、화생유、규화자유、도미유、감람유화지마유등시수식용유중감유삼지조성적보도。결과현시,감유삼지재상동식용유중적조성상사,이재불동식용유중적조성존재차이。채용본방법,통과차이적색보봉가직접식별출대두유중참입적5％저유。본방법위식별참가식용유제공료유개치적도경。
A reversed phase ultra performance liquid chromatography-mass spectrometric method was developed for the separation and analysis of triglycerides in edible oils. The samples were separated by using three ultra performance C18 columns in series with a total length of 40 cm (10 cm + 15 cm + 15 cm) at high pressure with acetonitrile-isopropanol (50:50, V/V) as mobile phase at a flow rate of 0. 2 mL/min and at col-umn temperature of 25℃, and detected by APCI ionization-mass spectrometry. The edible oil sample was dis-solved in isopropanol and injected in LC-MS directly. The triglycerides in edible oils were distinguished to their better fine components which included corn oil, peanut oil, sunflower seed oil, rice oil, olive oil, sesa-me oil and soybean oil. The chromatograms of different edible oils showed that the same kind of edible oil was composed of similar triglyceride composition and content, while the different kind of edible oils differed. The experimental result showed that the method could be use for identifying 5% lard adulterated in soybean oil. The method suggests a significant research way for identifying adulteration in edible oil.